NDLI: Design of transcriptional regulatory elements for Streptomyces

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Design of transcriptional regulatory elements for Streptomyces

Content Provider	Semantic Scholar
Author	Marques, Filipe Castro
Copyright Year	2015
Abstract	Streptomyces spp. are soil-dwelling bacteria well known for the ability to produce high added value metabolites for biotechnological application. The activation of secondary metabolites silent biosynthetic gene clusters encoded in Streptomyces genomes has been regarded as the renaissance of natural product discovery pipeline. Synthetic biology approaches are now been adopted for metabolic (re-)design and optimization. In this context, a diverse and well-characterized molecular toolbox is essential for construction and implementation of genetic circuits. The available constitutive promoters for Streptomyces are scarce and do not fulfil the standards of predictability and characterization needed for synthetic biology. In this work, publically available genome-wide transcriptomic and proteomic data of the model organism Streptomyces coelicolor A3(2) was used to obtain a short list of 12 stable and highly expressed genes. Further in silico analysis of the promoter regions of selected genes allowed to design three synthetic promoters (P1, P2 and P3). The designed promoters were characterized in Streptomyces coelicolor M145 and Streptomyces lividans 1326 in time-lapse studies, under heat-shock conditions and in defined carbon source media. This study provides proof-of-concept for a new strategy of promoter designing in Streptomyces.
File Format	PDF HTM / HTML
Alternate Webpage(s)	https://sigarra.up.pt/icbas/pt/pub_geral.show_file?pi_gdoc_id=64788
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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