NDLI: TPX2 overexpression in medullary thyroid carcinoma mediates TT cell proliferation.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yang, Xiaolin Liu, Geling Xiao, Hongzhen Yu, Fang Xiang, Xiuxiu Lu, Yifang Li, Weijuan Liu, Xiuling Li, Sha Shi, Yanping
Description	Country affiliation: China Author Affiliation: Yang X ( Department of Endocrinology (Section I), Tangshan Workers Hospital, Tangshan, China.)
Abstract	TPX2 (targeting protein for xenopus kinesin-like protein 2), a microtubule-associated protein, plays an important role in the formation of the mitotic spindle. Abnormal expression of TPX2 in various types of malignant tumors has been reported, but less is known for medullary thyroid cancer (MTC). We investigated the expression of TPX2 in human MTC tissues and its potential use as a therapeutic target. Immunohistochemical analysis of TPX2 expression was performed for 32 cases of MTC and 8 cases of normal thyroid. TPX2 expression was found to be significantly higher in MTC compared to normal thyroid tissues (P < 0.05), and to be associated with tumor size, lymph node metastasis, and advanced disease stage. The cellular effects of TPX2 knockdown, including cell proliferation, apoptosis, cell cycle diffusions, and mitotic gene expression were investigated using small interfering RNA (siRNA). TPX2-siRNA caused G1 and G2-phase cell cycle arrest, inhibited cell proliferation, and induced apoptosis. TPX2-siRNA also downregulated Aurora-A and cyclinB1 protein expression in MTC cells and enhanced the expression of p53 protein (P < 0.05). These results suggest that TPX2 may be of potential use as a new marker for MTC prognosis and therapy.
File Format	HTM / HTML
ISSN	12194956
Issue Number	3
Volume Number	20
e-ISSN	15322807
Journal	Pathology & Oncology Research
Language	English
Publisher	Springer
Publisher Date	2014-07-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Oncology Aurora Kinase A Metabolism Carcinoma, Medullary Secondary Cell Cycle Proteins Cell Proliferation Cyclin B1 Microtubule-associated Proteins Nuclear Proteins Thyroid Neoplasms Pathology Adult Apoptosis Genetics Blotting, Western Cell Cycle Checkpoints Antagonists & Inhibitors Female Flow Cytometry Gene Expression Regulation, Neoplastic Humans Immunoenzyme Techniques Lymphatic Metastasis Male Rna, Messenger Rna, Small Interfering Real-time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured Journal Article
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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