NDLI: PTEN and NEDD4 in Human Breast Carcinoma.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chen, Yilun Van De Vijver, Marc J. Hibshoosh, Hanina Parsons, Ramon Saal, Lao H.
Description	Country affiliation: Sweden Author Affiliation: Chen Y ( Division of Oncology and Pathology, Department of Clinical Sciences, Lund University, Lund, Sweden.); van de Vijver MJ ( Lund University Cancer Center, Lund, Sweden.); Hibshoosh H ( Department of Pathology, Academic Medical Center, Amsterdam, The Netherlands.); Parsons R ( Department of Pathology, Columbia University Medical Center, NY, USA.); Saal LH ( Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, NY, USA.)
Abstract	PTEN is an important tumor suppressor gene that antagonizes the oncogenic PI3K/AKT signaling pathway and has functions in the nucleus for maintaining genome integrity. Although PTEN inactivation by mutation is infrequent in breast cancer, transcript and protein levels are deficient in >25 % of cases. The E3 ubiquitin ligase NEDD4 (also known as NEDD4-1) has been reported to negatively regulate PTEN protein levels through poly-ubiquitination and proteolysis in carcinomas of the prostate, lung, and bladder, but its effect on PTEN in the breast has not been studied extensively. To investigate whether NEDD4 contributes to low PTEN levels in human breast cancer, we analyzed the expression of these proteins by immunohistochemistry across a large Swedish cohort of breast tumor specimens, and their transcript expression levels by microarrays. For both NEDD4 and PTEN, their transcript expression was significantly correlated to their protein expression. However, comparing NEDD4 expression to PTEN expression, either no association or a positive correlation was observed at the protein and transcript levels. This unexpected observation was further corroborated in two independent breast cancer cohorts from The Netherlands Cancer Institute and The Cancer Genome Atlas. Our results suggest that NEDD4 is not responsible for the frequent down-regulation of the PTEN protein in human breast carcinoma.
File Format	HTM / HTML
ISSN	12194956
e-ISSN	15322807
DOI	10.1007/s12253-015-9971-2
Journal	Pathology & Oncology Research
Issue Number	1
Volume Number	22
Language	English
Publisher	Springer
Publisher Date	2016-01-01
Publisher Place	Netherlands
Access Restriction	Open
Subject Keyword	Discipline Oncology Tumor Markers, Biological Metabolism Breast Neoplasms Endosomal Sorting Complexes Required For Transport Gene Expression Regulation, Neoplastic Pten Phosphohydrolase Ubiquitin-protein Ligases Genetics Pathology Cohort Studies Immunoenzyme Techniques Lymphatic Metastasis Neoplasm Staging Prognosis Rna, Messenger Real-time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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