| Author |
Cianci, Michele Bourenkov, Gleb Pompidor, Guillaume Karpics, Ivars Kallio, Johanna Bento, Isabel Roessle, Manfred Cipriani, Florent Fiedler, Stefan Schneider, Thomas R. |
| Description |
Country affiliation: Germany Author Affiliation: Cianci M ( Hamburg Unit c/o DESY, European Molecular Biology Laboratory (EMBL), Notkestrasse 85, 22603 Hamburg, Germany.); Bourenkov G ( Hamburg Unit c/o DESY, European Molecular Biology Laboratory (EMBL), Notkestrasse 85, 22603 Hamburg, Germany.); Pompidor G ( Hamburg Unit c/o DESY, European Molecular Biology Laboratory (EMBL), Notkestrasse 85, 22603 Hamburg, Germany.); Karpics I ( Hamburg Unit c/o DESY, European Molecular Biology Laboratory (EMBL), Notkestrasse 85, 22603 Hamburg, Germany.); Kallio J ( Hamburg Unit c/o DESY, European Molecular Biology Laboratory (EMBL), Notkestrasse 85, 22603 Hamburg, Germany.); Bento I ( Hamburg Unit c/o DESY, European Molecular Biology Laboratory (EMBL), Notkestrasse 85, 22603 Hamburg, Germany.); Roessle M ( Hamburg Unit c/o DESY, European Molecular Biology Laboratory (EMBL), Notkestrasse 85, 22603 Hamburg, Germany.); Cipriani F ( European Molecular Biology Laboratory, Institut Laue-Langevin, BP 181, 6 rue Jules Horowitz, 38042 Grenoble Cedex 9, France.); Fiedler S ( Hamburg Unit c/o DESY, European Molecular Biology Laboratory (EMBL), Notkestrasse 85, 22603 Hamburg, Germany.); Schneider TR ( Hamburg Unit c/o DESY, European Molecular Biology Laboratory (EMBL), Notkestrasse 85, 22603 Hamburg, Germany.) |
| Abstract |
The macromolecular crystallography P13 beamline is part of the European Molecular Biology Laboratory Integrated Facility for Structural Biology at PETRA III (DESY, Hamburg, Germany) and has been in user operation since mid-2013. P13 is tunable across the energy range from 4 to 17.5â
keV to support crystallographic data acquisition exploiting a wide range of elemental absorption edges for experimental phase determination. An adaptive Kirkpatrick-Baez focusing system provides an X-ray beam with a high photon flux and tunable focus size to adapt to diverse experimental situations. Data collections at energies as low as 4â
keV (λ = 3.1â
Å) are possible due to a beamline design minimizing background and maximizing photon flux particularly at low energy (up to 10â
photonsâ
s at 4â
keV), a custom calibration of the PILATUS 6M-F detector for use at low energies, and the availability of a helium path. At high energies, the high photon flux (5.4 × 10â
photonsâ
s at 17.5â
keV) combined with a large area detector mounted on a 2θ arm allows data collection to sub-atomic resolution (0.55â
Å). A peak flux of about 8.0 × 10â
photonsâ
s is reached at 11â
keV. Automated sample mounting is available by means of the robotic sample changer `MARVIN' with a dewar capacity of 160 samples. In close proximity to the beamline, laboratories have been set up for sample preparation and characterization; a laboratory specifically equipped for on-site heavy atom derivatization with a library of more than 150 compounds is available to beamline users. |
