NDLI: Development of a dose-limiting data collection strategy for serial synchrotron rotation crystallography.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hasegawa, Kazuya Yamashita, Keitaro Murai, Tomohiro Nuemket, Nipawan Hirata, Kunio Ueno, Go Ago, Hideo Nakatsu, Toru Kumasaka, Takashi Yamamoto, Masaki
Description	Country affiliation: Japan Author Affiliation: Hasegawa K ( Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo 679-5198, Japan.); Yamashita K ( RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo 679-5148, Japan.); Murai T ( Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, Kyoto 606-8501, Japan.); Nuemket N ( Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo 679-5198, Japan.); Hirata K ( RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo 679-5148, Japan.); Ueno G ( RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo 679-5148, Japan.); Ago H ( RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo 679-5148, Japan.); Nakatsu T ( RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo 679-5148, Japan.); Kumasaka T ( Japan Synchrotron Radiation Research Institute, 1-1-1 Kouto, Sayo 679-5198, Japan.); Yamamoto M ( RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo 679-5148, Japan.)
Abstract	Serial crystallography, in which single-shot diffraction images are collected, has great potential for protein microcrystallography. Although serial femtosecond crystallography (SFX) has been successfully demonstrated, limited beam time prevents its routine use. Inspired by SFX, serial synchrotron crystallography (SSX) has been investigated at synchrotron macromolecular crystallography beamlines. Unlike SFX, the longer exposure time of milliseconds to seconds commonly used in SSX causes radiation damage. However, in SSX, crystals can be rotated during the exposure, which can achieve efficient coverage of the reciprocal space. In this study, mercury single-wavelength anomalous diffraction (Hg-SAD) phasing of the luciferin regenerating enzyme (LRE) was performed using serial synchrotron rotation crystallography. The advantages of rotation and influence of dose on the data collected were evaluated. The results showed that sample rotation was effective for accurate data collection, and the optimum helical rotation step depended on multiple factors such as multiplicity and partiality of reflections, exposure time per rotation angle and the contribution from background scattering. For the LRE microcrystals, 0.25° was the best rotation step for the achievable resolution limit, whereas a rotation step larger than or equal to 1° was favorable for Hg-SAD phasing. Although an accumulated dose beyond 1.1âMGy caused specific damage at the Hg site, increases in resolution and anomalous signal were observed up to 3.4âMGy because of a higher signal-to-noise ratio.
File Format	HTM / HTML
ISSN	09090495
Issue Number	Pt 1
Journal	Journal of Synchrotron Radiation
Volume Number	24
e-ISSN	16005775
Language	English
Publisher	IUCr
Publisher Date	2017-01-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Radiology
Content Type	Text
Resource Type	Article
Subject	Nuclear and High Energy Physics Instrumentation Radiation

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