NDLI: Quantifiler® Trio Kit and forensic samples management: a matter of degradation.

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Quantifiler® Trio Kit and forensic samples management: a matter of degradation.

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Quantifiler® Trio Kit and forensic samples management: a matter of degradation.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Vernarecci, Stefano Ottaviani, Enrica Agostino, Alessandro Mei, Elisabetta Calandro, Lisa Montagna, Paola
Description	Country affiliation: Italy Author Affiliation: Vernarecci S ( Department of the Minister of Interior, Scientific Police Service, Forensic Genetics Laboratory, Rome, Italy.); Ottaviani E ( Department of the Minister of Interior, Scientific Police Service, Forensic Genetics Laboratory, Rome, Italy.); Agostino A ( Life Sciences Solutions, Thermo Fisher Scientific, Monza, Italy.); Mei E ( Department of the Minister of Interior, Scientific Police Service, Forensic Genetics Laboratory, Rome, Italy.); Calandro L ( Life Sciences Solutions, Thermo Fisher Scientific, South San Francisco, CA, USA.); Montagna P ( Department of the Minister of Interior, Scientific Police Service, Forensic Genetics Laboratory, Rome, Italy. Electronic address: paola.montagna@interno.it.)
Abstract	DNA collected from crime scenes may have experienced different levels of degradation. This is mainly due to sample exposure to different environmental factors. The impact of DNA degradation on short tandem repeat (STR) profiling can lead to partial or null information and in some cases, the identification of the trace may fail. The availability of a system enabling the assessment not only of the quantity of the DNA but also of its quality in terms of degradation would result in shorter time for sample processing, more reliable identifications and cost reduction by predicting the quality of the DNA profiles prior to STR analysis. We report here a study on 181 selected degraded DNA samples extracted from real crime scene evidence. The selected samples were processed by combining the use of a new commercial quantification kit (Quantifiler® Trio) with a new 24 marker multiplex PCR amplification kit (Globalfiler® Kit). Applying different statistical analyses we investigated the reliability of the Degradation Index provided by the Quantifiler® Trio in determining the level of DNA degradation in a forensic sample. This useful information can be used to predict the quality of the profile obtained after STR amplification. The combination of such a quantification kit with different PCR protocols allowed us to define practical guidelines for processing degraded forensic DNA samples with a simplified and comprehensive approach.
File Format	HTM / HTML
ISSN	18724973
Volume Number	16
e-ISSN	18780326
Journal	Forensic Science International: Genetics
Language	English
Publisher	Elsevier
Publisher Date	2015-05-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Forensic Sciences Dna Degradation, Necrotic Dna Fingerprinting Methods Forensic Genetics Reagent Kits, Diagnostic Instrumentation Humans Microsatellite Repeats Postmortem Changes Specimen Handling Evaluation Studies Journal Article
Content Type	Text
Resource Type	Article
Subject	Genetics Pathology and Forensic Medicine

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