NDLI: DNA transfer by examination tools--a risk for forensic casework?

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DNA transfer by examination tools--a risk for forensic casework?

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DNA transfer by examination tools--a risk for forensic casework?

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Szkuta, Bianca Harvey, Michelle L. Ballantyne, Kaye N. Van Oorschot, Roland A. H.
Description	Author Affiliation: Szkuta B ( School of Life and Environmental Sciences, Deakin University, 75 Pigdons Road, Waurn Ponds, Victoria 3216, Australia); Harvey ML ( School of Life and Environmental Sciences, Deakin University, 75 Pigdons Road, Waurn Ponds, Victoria 3216, Australia.); Ballantyne KN ( Office of the Chief Forensic Scientist, Victoria Police Forensic Services Department, 31 Forensic Drive, Macleod, Victoria 3085, Australia); van Oorschot RA ( Office of the Chief Forensic Scientist, Victoria Police Forensic Services Department, 31 Forensic Drive, Macleod, Victoria 3085, Australia.)
Abstract	The introduction of profiling systems with increased sensitivity has led to a concurrent increase in the risk of detecting contaminating DNA in forensic casework. To evaluate the contamination risk of tools used during exhibit examination we have assessed the occurrence and level of DNA transferred between mock casework exhibits, comprised of cotton or glass substrates, and high-risk vectors (scissors, forceps, and gloves). The subsequent impact of such transfer in the profiling of a target sample was also investigated. Dried blood or touch DNA, deposited on the primary substrate, was transferred via the vector to the secondary substrate, which was either DNA-free or contained a target sample (dried blood or touch DNA). Pairwise combinations of both heavy and light contact were applied by each vector in order to simulate various levels of contamination. The transfer of dried blood to DNA-free cotton was observed for all vectors and transfer scenarios, with transfer substantially lower when glass was the substrate. Overall touch DNA transferred less efficiently, with significantly lower transfer rates than blood when transferred to DNA-free cotton; the greatest transfer of touch DNA occurred between cotton and glass substrates. In the presence of a target sample, the detectability of transferred DNA decreased due to the presence of background DNA. Transfer had no impact on the detectability of the target profile, however, in casework scenarios where the suspect profiles are not known, profile interpretation becomes complicated by the addition of contaminating alleles and the probative value of the evidence may be affected. The results of this study reiterate the need for examiners to adhere to stringent laboratory cleaning protocols, particularly in the interest of contamination minimisation, and to reduce the handling of items to prevent intra-item transfer.
File Format	HTM / HTML
ISSN	18724973
Journal	Forensic Science International: Genetics
Volume Number	16
e-ISSN	18780326
Language	English
Publisher	Elsevier
Publisher Date	2015-05-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Forensic Sciences Dna Genetics Forensic Genetics Female Humans Male Journal Article
Content Type	Text
Resource Type	Article
Subject	Genetics Pathology and Forensic Medicine

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