NDLI: Developmental validation of a fully integrated sample-to-profile rapid human identification system for processing single-source reference buccal samples.

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Developmental validation of a fully integrated sample-to-profile rapid human identification system for processing single-source reference buccal samples.

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Developmental validation of a fully integrated sample-to-profile rapid human identification system for processing single-source reference buccal samples.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Jovanovich, Stevan Bogdan, Greg Belcinski, Richard Buscaino, Jacklyn Burgi, Dean Butts, Erica L. R. Chear, Kaiwan Ciopyk, Brian Eberhart, David El-Sissi, Omar Franklin, Helen Gangano, Stefanie Gass, Jennifer Harris, Dennis Hennessy, Lori Kindwall, Alex King, David Klevenberg, Jim Li, Yuan Mehendale, Neelima McIntosh, Roger Nielsen, Bill Park, Charles Pearson, Francesca Schueren, Robert Stainton, Nancy Troup, Charles Vallone, Peter M. Vangbo, Mattias Woudenberg, Timothy Wyrick, David Williams, Stephen
Description	Author Affiliation: Jovanovich S ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA. Electronic address: stevanj@integenx.com.); Bogdan G ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Belcinski R ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Buscaino J ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Burgi D ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Butts EL ( National Institute of Standards and Technology, Gaithersburg, MD 20899-8314, USA.); Chear K ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Ciopyk B ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Eberhart D ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); El-Sissi O ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Franklin H ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Gangano S ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Gass J ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Harris D ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Hennessy L ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Kindwall A ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); King D ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Klevenberg J ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Li Y ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Mehendale N ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); McIntosh R ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Nielsen B ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Park C ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Pearson F ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Schueren R ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Stainton N ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Troup C ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Vallone PM ( National Institute of Standards and Technology, Gaithersburg, MD 20899-8314, USA.); Vangbo M ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Woudenberg T ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Wyrick D ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.); Williams S ( IntegenX Inc. 5720 Stoneridge Drive, Suite 300, Pleasanton, CA 94588-2739, USA.)
Abstract	UNLABELLED: Short tandem repeat (STR) DNA typing is a global standard for human identification. Current practice involves highly trained forensic analysts, operating in a laboratory setting, using multiple instruments to process samples and analyze the data. Here, we report the developmental validation of a fully integrated and automated DNA profiling system, the RapidHIT® System, capable of producing up to five high quality STR profiles with full controls in approximately 90min using PowerPlex®16 HS RapidHIT chemistry. The system integrates all sample handling steps: starting from lysis of cells on buccal swabs or other buccal sample types through DNA extraction, normalization, amplification,capillary array electrophoresis, detection, and integrated software analysis. The results describe the developmental validation of the RapidHIT™ System for buccal samples processed with the DNA IQ™ extraction chemistry using a guandinium chaotropic agent and paramagnetic beads followed by amplification using a modified version of PowerPlex 16 HS chemistry (PowerPlex 16 HS RapidHIT chemistry), and capillary electrophoresis with manual review of genotyping data following interpretation guidelines. All processing from the buccal swab to generation and processing of the profile occurs on the RapidHIT platform. RESULT: are concordant with traditional methods, with 88% first pass success rates for both the CODIS and PowerPlex 16 loci. Average peak height ratios were 0.89 for buccal swabs. The system produces full profiles from swabs with at least 176 ng of saliva DNA. Rapid DNA identification systems will significantly enhance capabilities for forensic labs, intelligence, defense, law enforcement, refugee and immigration applications, and kinship analysis.
File Format	HTM / HTML
ISSN	18724973
Journal	Forensic Science International: Genetics
Volume Number	16
e-ISSN	18780326
Language	English
Publisher	Elsevier
Publisher Date	2015-05-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Forensic Sciences Forensic Anthropology Mouth Mucosa Metabolism Humans Microsatellite Repeats Genetics Polymerase Chain Reaction Reproducibility Of Results Journal Article Validation Studies
Content Type	Text
Resource Type	Article
Subject	Genetics Pathology and Forensic Medicine

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Reduction of Powerplex(®) Y23 reaction volume for genotyping buccal cell samples on FTA(TM) cards.

Optimization and validation of a fast amplification protocol for AmpFlSTR® Profiler Plus® for rapid forensic human identification.

Evaluation of the RapidHIT™ 200, an automated human identification system for STR analysis of single source samples.

Developmental validation of the PowerPlex® Y23 System: a single multiplex Y-STR analysis system for casework and database samples.

Performance of Identifiler Direct and PowerPlex 16 HS on the Applied Biosystems 3730 DNA Analyzer for processing biological samples archived on FTA cards.

Developmental validation of the PowerPlex® 18D System, a rapid STR multiplex for analysis of reference samples.

Developmental validation of the EX20+4 system.

Developmental validation of a forensic rapid DNA-STR kit: Expressmarker 16.

I-DNASE21 system: development and SWGDAM validation of a new STR 21-plex reaction.

Developmental validation of a fully integrated sample-to-profile rapid human identification system for processing single-source reference buccal samples.

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Reduction of Powerplex(®) Y23 reaction volume for genotyping buccal cell samples on FTA(TM) cards.

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Evaluation of the RapidHIT™ 200, an automated human identification system for STR analysis of single source samples.

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Developmental validation of a fully integrated sample-to-profile rapid human identification system for processing single-source reference buccal samples.