NDLI: ESDA®-Lite collection of DNA from latent fingerprints on documents.

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ESDA®-Lite collection of DNA from latent fingerprints on documents.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Plaza, Dane T. Mealy, Jamia L. Lane, J. Nicholas Parsons, M. Neal Bathrick, Abigail S. Slack, Donia P.
Description	Author Affiliation: Plaza DT ( The Bode Technology Group, 10430 Furnace Road, Suite 107, Lorton, VA 22079, USA. Electronic address: dane.plaza@bodetech.com.); Mealy JL ( The Bode Technology Group, 10430 Furnace Road, Suite 107, Lorton, VA 22079, USA.); Lane JN ( The Bode Technology Group, 10430 Furnace Road, Suite 107, Lorton, VA 22079, USA.); Parsons MN ( The Bode Technology Group, 10430 Furnace Road, Suite 107, Lorton, VA 22079, USA.); Bathrick AS ( The Bode Technology Group, 10430 Furnace Road, Suite 107, Lorton, VA 22079, USA.); Slack DP ( The Bode Technology Group, 10430 Furnace Road, Suite 107, Lorton, VA 22079, USA.)
Abstract	The ability to detect and non-destructively collect biological samples for DNA processing would benefit the forensic community by preserving the physical integrity of evidentiary items for more thorough evaluations by other forensic disciplines. The Electrostatic Detection Apparatus (ESDA®) was systemically evaluated for its ability to non-destructively collect DNA from latent fingerprints deposited on various paper substrates for short tandem repeat (STR) DNA profiling. Fingerprints were deposited on a variety of paper substrates that included resume paper, cotton paper, magazine paper, currency, copy paper, and newspaper. Three DNA collection techniques were performed: ESDA collection, dry swabbing, and substrate cutting. Efficacy of each collection technique was evaluated by the quantity of DNA present in each sample and the percent profile generated by each sample. Both the ESDA and dry swabbing non-destructive sampling techniques outperformed the destructive methodology of substrate cutting. A greater number of full profiles were generated from samples collected with the non-destructive dry swabbing collection technique than were generated from samples collected with the ESDA; however, the ESDA also allowed the user to visualize the area of interest while non-destructively collecting the biological material. The ability to visualize the biological material made sampling straightforward and eliminated the need for numerous, random swabbings/cuttings. Based on these results, the evaluated non-destructive ESDA collection technique has great potential for real-world forensic implementation.
File Format	HTM / HTML
ISSN	18724973
Volume Number	16
e-ISSN	18780326
Journal	Forensic Science International: Genetics
Language	English
Publisher	Elsevier
Publisher Date	2015-05-01
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Forensic Sciences Dna Fingerprinting Methods Dna Isolation & Purification Dermatoglyphics Analysis Genetics Forensic Genetics Humans Microsatellite Repeats Paper Specimen Handling Static Electricity Evaluation Studies Journal Article Research Support, U.s. Gov't, Non-p.h.s.
Content Type	Text
Resource Type	Article
Subject	Genetics Pathology and Forensic Medicine

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