NDLI: Generation of induced pluripotent stem cells from human adipose-derived stem cells without c-MYC.

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Generation of induced pluripotent stem cells from human adipose-derived stem cells without c-MYC.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Aoki, Tetsuhiro Ohnishi, Hiroe Oda, Yasuaki Tadokoro, Mika Sasao, Mari Kato, Hiroyuki Hattori, Koji Ohgushi, Hajime
Description	Country affiliation: Japan Author Affiliation: Aoki T ( Tissue Engineering Research Group, Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Amagasaki, Hyogo, Japan.)
Abstract	Human adipose-derived stem cells (hASCs) are ubiquitous, plentiful, and easily/safely obtainable cells derived from adipose tissue, regardless of the age and sex of the donor. However, the hASCs have limited proliferative and differentiation capabilities. In this study, we examined whether induced pluripotent stem cells (iPSCs) could be generated from hASCs. We transduced hASCs with three human transcription factors (OCT3/4, SOX2, and KLF4), and found that they formed human embryonic stem cell (ESC)-like colonies. Importantly, we did not transduce c-MYC, which is usually utilized to generate iPSCs but is considered an oncogene. These colonies expressed human ESC-specific surface antigens (stage-specific embryonic antigens SSEA-3 and SSEA-4, and tumor-related antigens TRA-1-60 and TRA-1-81), endogenous transcription factors (OCT3/4, NANOG, and SOX2), and undifferentiated human ESC marker genes (REX1, UTF1, GDF3, DPPA2, DPPA4, and DPPA5). Further, the colonies were able to differentiate into the three germ layers both in vitro and in vivo. These results show that human iPSCs can be generated by the transduction of three factors (OCT3/4, SOX2, and KLF4) into hASCs.
File Format	HTM / HTML
ISSN	21524947
Issue Number	7
Volume Number	16
e-ISSN	21524955
Journal	Tissue Engineering Part A
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2010-07-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Biotechnology Adipose Tissue Cytology Cell Culture Techniques Methods Induced Pluripotent Stem Cells Proto-oncogene Proteins C-myc Metabolism Animals Cell Differentiation Cell Line Embryo, Mammalian Embryonic Stem Cells Gene Expression Regulation Humans Karyotyping Mice Microsatellite Repeats Genetics Reverse Transcriptase Polymerase Chain Reaction Teratoma Pathology Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article

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Human induced pluripotent stem cells derived from fetal neural stem cells successfully undergo directed differentiation into cartilage.

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Generation of induced pluripotent stem cells from human adipose-derived stem cells without c-MYC.

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