NDLI: A new animal model for assessing cartilage repair and regeneration at a nonarticular site.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Moyer, Hunter R. Wang, Yun Farooque, Tanya Wick, Timothy Singh, Kimberly A. Xie, Liqin Guldberg, Robert E. Williams, Joseph K. Boyan, Barbara D. Schwartz, Zvi
Description	Country affiliation: United States Author Affiliation: Moyer HR ( Division of Plastic and Reconstructive Surgery, Department of Surgery, Emory University School of Medicine, Atlanta, Georgia, USA.)
Abstract	The aim of this study was to establish a critical-sized nonjoint chondral defect animal model and to evaluate its feasibility for testing cartilage regeneration strategies. Dermal biopsy punches 1-4 mm in diameter were used to create cylindrical full-thickness defects in the center of athymic rat xiphoids. The 3 and 4 mm defects remained unhealed 35 days postsurgery, with a large area in the center that had low proteoglycan content based on contrast-enhanced microCT (EPIC-microCT), radiographic, and histological analyses. In a second step, tissue-engineered cartilage was synthesized by culturing primary bovine articular chondrocytes on poly-L-lactic acid (PLA) scaffolds in a perfusion-shear bioreactor for 28 days. These chondrocyte/PLA constructs or primary bovine chondrocytes were implanted into 3-mm-diameter defects. Empty defects and defects implanted with empty PLA scaffolds were used as controls. Xiphoids were harvested 28 days after surgery and examined with faxitron, microCT, and histology using hematoxylin and eosin and safranin-O staining. Both chondrocyte/PLA constructs and chondrocytes alone formed neocartilage. The results indicate that a 3 mm cylindrical defect in a rat xiphoid is an economic, feasible, and reproductive model to evaluate the potential of various constructs for nonjoint cartilage repair.
File Format	HTM / HTML
ISSN	21524947
Issue Number	7
Volume Number	16
e-ISSN	21524955
Journal	Tissue Engineering Part A
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2010-07-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Biotechnology Cartilage, Articular Physiology Models, Animal Regeneration Wound Healing Xiphoid Bone Pathology Animals Surgery Cattle Glycosaminoglycans Metabolism Male Rats X-ray Microtomography Journal Article Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s.
Content Type	Text
Resource Type	Article

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