NDLI: The role of endothelial progenitor cells in prevascularized bone tissue engineering: development of heterogeneous constructs.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Fedorovich, Natalja E. Haverslag, René T. Dhert, Wouter J. A. Alblas, Jacqueline
Description	Country affiliation: Netherlands Author Affiliation: Fedorovich NE ( Department of Orthopaedics, University Medical Center Utrecht, The Netherlands.)
Abstract	In vitro prevascularization of bone grafts with endothelial progenitor cells (EPCs) is a promising strategy to improveimplant survival. In this study we show bone formation in constructs that contain multipotent stromal cells (MSCs) and EPCs. Early and late EPCs from peripheral blood and bone marrow of adult goats were characterized for differentiation markers and functional responses. EPCs from peripheral blood are more proliferative than bone-marrow-derived EPCs, express higher numbers of endothelial markers for longer periods of time, and form more intricate networks. We demonstrate that EPCs derived from peripheral blood contribute to osteogenic differentiation by MSCs in vitro, and that MSCs support the proliferation of EPCs and stabilize the formed cellular networks. In vivo, EPCs from peripheral blood assemble into early blood vessel networks, which are more pronounced in the presence of MSCs. These results show that the EPCs isolated from peripheral blood are suitable for prevascularization strategies, and that coseeding of EPCs and MSCs is favorable for bone formation after 6 weeks.
File Format	HTM / HTML
ISSN	21524947
Issue Number	7
Volume Number	16
e-ISSN	21524955
Journal	Tissue Engineering Part A
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2010-07-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Biotechnology Bone And Bones Blood Supply Endothelial Cells Cytology Neovascularization, Physiologic Stem Cells Tissue Engineering Methods Tissue Scaffolds Chemistry Animals Biological Assay Biological Markers Metabolism Bone Marrow Cells Drug Effects Cell Differentiation Cell Proliferation Cell Separation Coculture Techniques Collagen Pharmacology Colony-forming Units Assay Drug Combinations Goats Humans Laminin Mice Mice, Nude Osteogenesis Proteoglycans Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article

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