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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Matsuse, Dai Nakahata, Tatsutoshi Kitada, Masaaki Dezawa, Mari Wakao, Shohei Kikuchi, Tomohiko Kuroda, Yasumasa Shigemoto, Taeko Tanimura, Yukihiro Tsuchiyama, Kenichiro Fujiyoshi, Yoshinori Goda, Makoto Akashi, Hideo |
| Description | Author Affiliation: Wakao S ( Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine, Sendai, Miyagi 980-8575, Japan.); |
| Abstract | The stochastic and elite models have been proposed for the mechanism of induced pluripotent stem (iPS) cell generation. In this study we report a system that supports the elite model. We previously identified multilineage-differentiating stress-enduring (Muse) cells in human dermal fibroblasts that are characterized by stress tolerance, expression of pluripotency markers, self-renewal, and the ability to differentiate into endodermal-, mesodermal-, and ectodermal-lineage cells from a single cell. They can be isolated as stage-specific embryonic antigen-3/CD105 double-positive cells. When human fibroblasts were separated into Muse and non-Muse cells and transduced with Oct3/4, Sox2, Klf4, and c-Myc, iPS cells were generated exclusively from Muse cells but not from non-Muse cells. Although some colonies were formed from non-Muse cells, they were unlike iPS cells. Furthermore, epigenetic alterations were not seen, and some of the major pluripotency markers were not expressed for the entire period during iPS cell generation. These findings were confirmed further using cells transduced with a single polycistronic virus vector encoding all four factors. The results demonstrate that in adult human fibroblasts a subset of preexisting adult stem cells whose properties are similar in some respects to those of iPS cells selectively become iPS cells, but the remaining cells make no contribution to the generation of iPS cells. Therefore this system seems to fit the elite model rather than the stochastic model. |
| ISSN | 00278424 |
| e-ISSN | 10916490 |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Issue Number | 24 |
| Volume Number | 108 |
| Language | English |
| Publisher | National Academy of Sciences |
| Publisher Date | 2011-06-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Cell Differentiation Cell Lineage Fibroblasts Cytology Induced Pluripotent Stem Cells Adaptation, Physiological Animals Antigens, CD Metabolism Antigens, Surface Antigens, Tumor-Associated, Carbohydrate Cell Line Cells, Cultured Dermis Immunohistochemistry Kruppel-Like Transcription Factors Genetics Mice Mice, Inbred NOD Mice, SCID Octamer Transcription Factor-3 Proto-Oncogene Proteins C-myc Receptors, Cell Surface Reverse Transcriptase Polymerase Chain Reaction SOXB1 Transcription Factors Stage-Specific Embryonic Antigens Stress, Physiological Transfection Research Support, Non-U.S. Gov't Multidisciplinary |
| Content Type | Text |
| Resource Type | Article |
| Subject | Multidisciplinary |
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