NDLI: Reprogramming of somatic cells after fusion with induced pluripotent stem cells and nuclear transfer embryonic stem cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sumer, Huseyin Jones, Karen L. Liu, Jun Heffernan, Corey Tat, Pollyanna A. Upton, Kyle R. Verma, Paul J.
Description	Country affiliation: Australia Author Affiliation: Sumer H ( Monash Institute of Medical Research, Monash University, Clayton, Victoria, Australia. huseyin.sumer@med.monash.edu.au)
Abstract	In this study we examine whether a somatic cell, once returned to a pluripotent state, gains the ability to reprogram other somatic cells. We reprogrammed mouse embryonic fibroblasts by viral induction of oct4, sox2, c-myc, and klf-4 genes. Upon fusion of the resulting iPS cells with somatic cells harboring an Oct4-GFP transgene we observed, GFP expression along with activation of Oct4 from the somatic genome, expression of key pluripotency genes, and positive immunostaining for Oct4, SSEA-1, and alkaline phosphatase. The iPS-somatic hybrids had the ability to differentiate into cell types indicative of the three germ layers and were able to localize to the inner cell mass of aggregated embryos. Furthermore, ntES cells were used as fusion partners to generate hybrids, which were also confirmed to be reprogrammed to a pluripotent state. These results demonstrate that once a somatic cell nucleus is reprogrammed, it acquires the capacity and potency to reprogram other somatic cells by cell fusion and shares this functional property with normal embryonic stem (ES) cells.
File Format	HTM / HTML
ISSN	15473287
Issue Number	2
Volume Number	19
e-ISSN	15578534
Journal	Stem Cells and Development
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2010-02-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Cell Biology Discipline Hematology Embryonic Stem Cells Cytology Fibroblasts Hybrid Cells Induced Pluripotent Stem Cells Animals Cell Fusion Cell Transplantation Methods Cells, Cultured Cellular Reprogramming Embryo, Mammalian Metabolism Female Green Fluorescent Proteins Genetics Immunohistochemistry Transplantation Male Mice Mice, Scid Mice, Transgenic Octamer Transcription Factor-3 Pregnancy Reverse Transcriptase Polymerase Chain Reaction Teratoma Pathology Transduction, Genetic Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Hematology

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