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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Nakayama, Karina H. Batchelder, Cynthia A. Lee, Chang I. Tarantal, Alice F. |
| Description | Country affiliation: United States Author Affiliation: Nakayama KH ( Center of Excellence in Translational Human Stem Cell Research, California National Primate Research Center, Davis, California 95616-8542, USA.) |
| Abstract | The goal of this study was the production of a decellularized kidney scaffold with structural, mechanical, and physiological properties necessary for engineering basic renal structures in vitro. Fetal, infant, juvenile, and adult rhesus monkey kidney sections were treated with either 1% (v/v) sodium dodecyl sulfate or Triton X-100 followed by quantitative and qualitative analysis. Comparison of decellularization agents and incubation temperatures demonstrated sodium dodecyl sulfate at 4 degrees C to be most effective in preserving the native architecture. Hematoxylin and eosin staining confirmed the removal of cellular material, and immunohistochemistry demonstrated preservation of native expression patterns of extracellular matrix proteins, including heparan sulfate proteoglycan, fibronectin, collagen types I and IV, and laminin. Biomechanical testing revealed a decrease in the compressive modulus of decellularized compared to fresh kidneys. Layering of fetal kidney explants on age-matched decellularized kidney scaffolds demonstrated the capacity of the scaffold to support Pax2+/vimentin+ cell attachment and migration to recellularize the scaffold. These findings demonstrate that decellularized kidney sections retain critical structural and functional properties necessary for use as a three-dimensional scaffold and promote cellular repopulation. Further, this study provides the initial steps in developing new regenerative medicine strategies for renal tissue engineering and repair. |
| File Format | HTM / HTML |
| ISSN | 21524947 |
| e-ISSN | 21524955 |
| DOI | 10.1089/ten.TEA.2009.0602 |
| Journal | Tissue Engineering Part A |
| Issue Number | 7 |
| Volume Number | 16 |
| Language | English |
| Publisher | Mary Ann Liebert, Inc. |
| Publisher Date | 2010-07-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Biotechnology Cell Culture Techniques Kidney Cytology Tissue Engineering Tissue Scaffolds Chemistry Animals Biomechanical Phenomena Extracellular Matrix Proteins Metabolism Fluorescent Antibody Technique Macaca Mulatta Organ Size Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
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