NDLI: Cluster of differentiation antibody microarrays on plasma immersion ion implanted polycarbonate.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kosobrodova, E. Mohamed, A. Su, Y. Kondyurin, A. Dos Remedios, C. G. McKenzie, D. R. Bilek, M. M. M.
Description	Author Affiliation: Kosobrodova E ( Department of Applied Plasma and Physics, School of Physics, University of Sydney, Sydney, 2006, Australia. Electronic address: elenak@physics.usyd.edu.au.); Mohamed A ( Muscle Research Unit, Discipline of Anatomy and Histology, Bosch Institute, University of Sydney, Sydney, 2006, Australia.); Su Y ( Australian Center of Microscopy and Analysis, University of Sydney, Sydney, 2006, Australia.); Kondyurin A ( Department of Applied Plasma and Physics, School of Physics, University of Sydney, Sydney, 2006, Australia.); dos Remedios CG ( Muscle Research Unit, Discipline of Anatomy and Histology, Bosch Institute, University of Sydney, Sydney, 2006, Australia.); McKenzie DR ( Department of Applied Plasma and Physics, School of Physics, University of Sydney, Sydney, 2006, Australia.); Bilek MM ( Department of Applied Plasma and Physics, School of Physics, University of Sydney, Sydney, 2006, Australia.)
Abstract	Plasma immersion ion implantation (PIII) modifies the surface properties of polymers, enabling them to covalently immobilize proteins without using linker chemistry. We describe the use of PIII treated polycarbonate (PC) slides as a novel platform for producing microarrays of cluster of differentiation (CD) antibodies. We compare their performance to identical antibody microarrays printed on nitrocellulose-coated glass slides that are currently the industry standard. Populations of leukocytes are applied to the CD microarrays and unbound cells are removed revealing patterns of differentially immobilized cells that are detected in a simple label-free approach by scanning the slides with visible light. Intra-slide and inter-slide reproducibility, densities of bound cells, and limits of detection were determined. Compared to the nitrocellulose-coated glass slides, PIII treated PC slides have a lower background noise, better sensitivity, and comparable or better reproducibility. They require three-fold lower antibody concentrations to yield equivalent signal strength, resulting in significant reductions in production cost. The improved transparency of PIII treated PC in the near-UV and visible wavelengths combined with superior immobilization of biomolecules makes them an attractive platform for a wide range of microarray applications.
File Format	HTM / HTML
ISSN	09284931
Volume Number	35
e-ISSN	18730191
Journal	Materials Science and Engineering: C
Language	English
Publisher	Elsevier
Publisher Date	2014-02-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Materials Science Antigens, Cd Immunology Immunoassay Instrumentation Plasma Gases Chemistry Polycarboxylate Cement Protein Array Analysis Refractometry Analysis Equipment Design Equipment Failure Analysis Ions Radiation Effects Spectroscopy, Fourier Transform Infrared Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Biomaterials Condensed Matter Physics Bioengineering Mechanical Engineering

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