NDLI: Effect of cold working on biocompatibility of Ni-free high nitrogen austenitic stainless steels using Dalton's Lymphoma cell line.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Talha, Mohd Kumar, Sanjay Behera, C. K. Sinha, O. P.
Description	Country affiliation: India Author Affiliation: Talha M ( Centre of Advanced Study, Department of Metallurgical Engineering, Indian Institute of Technology (Banaras Hindu University) Varanasi 221005, Uttar Pradesh, India.); Kumar S ( Centre of Advanced Study, Department of Zoology, Banaras Hindu University, Varanasi 221005, Uttar Pradesh, India.); Behera CK ( Centre of Advanced Study, Department of Metallurgical Engineering, Indian Institute of Technology (Banaras Hindu University) Varanasi 221005, Uttar Pradesh, India.); Sinha OP ( Centre of Advanced Study, Department of Metallurgical Engineering, Indian Institute of Technology (Banaras Hindu University) Varanasi 221005, Uttar Pradesh, India. Electronic address: opsinha.met@itbhu.ac.in.)
Abstract	The aims of the present work are to explore the effect of cold working on in-vitro biocompatibility of indigenized low cost Ni-free nitrogen containing austenitic stainless steels (HNSs) and to compare it with conventionally used biomedical grade, i.e. AISI 316L and 316LVM, using Dalton's Lymphoma (DL) cell line. The MTT assay [3-(4,5-dimethythiazol 2-yl)-2,5-diphenyltetrazolium bromide] was performed on DL cell line for cytotoxicity evaluation and cell adhesion test. As a result, it was observed that the HNS had higher cell proliferation and cell growth and it increases by increasing nitrogen content and degree of cold working. The surface wettability of the alloys was also investigated by water contact angle measurements. The value of contact angles was found to decrease with increase in nitrogen content and degree of cold working. This indicates that the hydrophilic character increases with increasing nitrogen content and degree of cold working which further attributed to enhance the surface free energy (SFE) which would be conducive to cell adhesion which in turn increases the cell proliferation.
File Format	HTM / HTML
ISSN	09284931
Volume Number	35
e-ISSN	18730191
Journal	Materials Science and Engineering: C
Language	English
Publisher	Elsevier
Publisher Date	2014-02-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Materials Science Biocompatible Materials Chemical Synthesis Pharmacology Lymphoma Pathology Physiopathology Stainless Steel Chemistry Cell Line, Tumor Cell Proliferation Drug Effects Cell Survival Cold Temperature Hardness Humans Materials Testing Nickel Isolation & Purification Nitrogen Comparative Study Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Mechanics of Materials Biomaterials Condensed Matter Physics Bioengineering Mechanical Engineering

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