NDLI: Development and validation of H11B2C2 monoclonal antibody-reactive hyaluronic acid binding protein: overexpression of HABP during human tumor progression

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Boregowda, Rajeev K. Appaiah, Hitesh N. Karunakumar, Mortha Parameshwariah, Shivanna Avadani, Geetha Sunila, Sunila Banerjee, Shibdas
Description	Country affiliation: United States Author Affiliation: Boregowda RK ( Department of Medicine, Division of Medical Oncology, UMDNJ-The Robert Wood Johnson Medical School, The Cancer Institute of New Jersey, 195 Little Albany Street, New Brunswick, NJ 08903, USA. rajeev04mys@yahoo.com)
Abstract	Informative biomarkers of tumor progression have been elusive. The interaction between hyaluronic acid (HA) and its binding proteins (HABP) plays a pivotal role during malignancy. In the present study, we have developed a monoclonal antibody (mAb, termed as H11B2C2 mAb) and showed that this mAb specifically reacts with overexpressed HABP from a wide variety of malignant tumors as compared with benign tumors. In Western blot analysis, H11B2C2 mAb detected a major 80-kDa protein from human cancer cell lines, and the overexpression of 55–57- and 30-kDa proteins in malignant tumors compared with benign tumors. Furthermore, immunohistochemical analysis of different types of benign and malignant tumors with different grades showed higher expression of HABP in all the malignant tumors when compared with the benign tumors. HABP overexpression was specific to tumor cells when compared with the surrounding stroma and localized on the cell surface as well as in the intracellular region. The competitive inhibition experiments using HA polymer and its oligosaccharides in the Western blot and immunohistopathology experiments suggested that the H11B2C2 mAb-reactive protein is HABP. Altogether, the present study showed overexpression of the H11B2C2 mAb-reactive HABP in various malignant tumors as compared with benign tumors. Thus, H11B2C2 mAb-reactive HABP can be used as a potential biomarker during tumor progression.
File Format	HTM / HTML
ISSN	10104283
Issue Number	1
Volume Number	34
e-ISSN	14230380
Journal	Tumor Biology
Language	English
Publisher	Springer
Publisher Date	2013-02-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Medicine__semicolon__oncology Antibodies, Monoclonal Immunology Antigens, Cd44 Biosynthesis Neoplasms Metabolism Tumor Markers, Biological Cell Line, Tumor Cell Membrane Disease Progression Female Humans Hyaluronic Acid Male Pathology Journal Article
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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