NDLI: VEGF-C expression in oral cancer by neurotransmitter-induced activation of beta-adrenergic receptors.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Vilardi, Bruna Maria Rodrigues Bravo-Calderón, Diego Mauricio Bernabé, Daniel Galera Oliveira, Sandra Helena Penha Oliveira, Denise Tostes
Description	Country affiliation: Brazil Author Affiliation: Vilardi BM ( Department of Stomatology, Area of Pathology, Bauru School of Dentistry, University of São Paulo, Alameda Octávio Pinheiro Brisolla, 9-75, 17012-901, Bauru, São Paulo, Brazil.)
Abstract	The aim of this study was to investigate the expression of vascular endothelial growth factor type C (VEGF-C) in oral squamous cell carcinoma (OSCC) cell lines through norepinephrine-induced activation of beta-adrenergic receptors. Human OSCC cell lines (SCC-9 and SCC-25) expressing beta-adrenergic receptors were stimulated with different concentrations of norepinephrine (0.1, 1, and 10 µM) and 1 µM of propranolol, and analyzed after 1, 6, and 24 h. VEGF-C gene expression and VEGF-C production in the cell supernatant were evaluated by real-time PCR and by ELISA, respectively. The results showed that beta-adrenergic receptor stimulation by different concentrations of norepinephrine or blocking by propranolol did not markedly alter VEGF-C expression by SCC-9 and SCC-25 cells. VEGF-C protein levels produced by oral malignant cell lines after stimulation with different norepinephrine concentrations or blocking with propranolol was statistically similar (p > 0.05) to those of the control group (nonstimulated OSCC cell lines). Our findings suggest that stimulation of beta-adrenergic receptors by means of norepinephrine does not seem to modulate the VEGF-C expression in OSCC cell lines. These findings reinforce the need for further studies in order to understand the responsiveness of oral cancer to beta-adrenergic receptor stimulation or blockage, especially with regard to VEGF-C production.
File Format	HTM / HTML
ISSN	10104283
Issue Number	1
Volume Number	34
e-ISSN	14230380
Journal	Tumor Biology
Language	English
Publisher	Springer
Publisher Date	2013-02-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Medicine__semicolon__oncology Carcinoma, Squamous Cell Metabolism Mouth Neoplasms Norepinephrine Propranolol Pharmacology Receptors, Adrenergic, Beta Vascular Endothelial Growth Factor C Adrenergic Beta-antagonists Cell Line, Tumor Humans Neurotransmitter Agents Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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