NDLI: Berberine inhibits the migration and invasion of T24 bladder cancer cells via reducing the expression of heparanase.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yan, Lei Yan, Keqiang Kun, Wang Xu, Lin Ma, Qian Tang, Yueqing Jiao, Wei Gu, Gangli Fan, Yidon Xu, Zhonghua
Description	Country affiliation: China Author Affiliation: Yan L ( Shandong University School of Medicine, Jinan, 250012, China.)
Abstract	Berberine, a quaternary amine and isoquinoline alkaloid, has been proposed to have antimetastatic effects on many types of tumor cells; however, its exact inhibitory mechanisms with bladder cancer cells remain unclear. We used berberine and siRNA technology to interfere the expression of heparanase in bladder cancer T24 cells, detected the expression of heparanase mRNA and protein by reverse transcription PCR and Western blot respectively, and investigated their effects on the migration and invasion of T24 bladder cancer cells using transwell chamber. Our results showed that both mRNA and protein of heparanase were highly expressed in human bladder cancer T24 cells and markedly downregulated by both heparanase-specific siRNA (hpa-siRNA) and berberine. The tumor cell migration assay indicated that transfection of hpa-siRNA and treatment with berberine both attenuated the migration and invasion of T24 cells. Therefore, berberine inhibits the metastasis and invasion of bladder cancer cell, possibly via blocking the heparanase expression and thus may be used clinically to reduce the recurrence of bladder cancer.
File Format	HTM / HTML
ISSN	10104283
Issue Number	1
Volume Number	34
e-ISSN	14230380
Journal	Tumor Biology
Language	English
Publisher	Springer
Publisher Date	2013-02-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Medicine__semicolon__oncology Berberine Pharmacology Glucuronidase Biosynthesis Urinary Bladder Neoplasms Drug Therapy Pathology Antineoplastic Agents Cell Line, Tumor Cell Movement Drug Effects Genetics Humans Neoplasm Invasiveness Rna Interference Rna, Messenger Metabolism Rna, Small Interfering Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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