NDLI: Adenosine induces cell cycle arrest and apoptosis via cyclinD1/Cdk4 and Bcl-2/Bax pathways in human ovarian cancer cell line OVCAR-3.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Shirali, Saeid Aghaei, Mahmoud Shabani, Mahdi Fathi, Mojtaba Sohrabi, Majid Moeinifard, Marzieh
Description	Country affiliation: Iran Author Affiliation: Shirali S ( Department of Laboratory Sciences, Chalous Branch, Islamic Azad University, Chalous, Iran.)
Abstract	Adenosine is a regulatory molecule with widespread physiological effects in almost every cells and acts as a potent regulator of cell growth. Adenosine has been shown to inhibit cell growth and induce apoptosis in the several cancer cells via caspase activation and Bcl-2/Bax pathway. The present study was designed to understand the mechanism underlying adenosine-induced apoptosis in the OVCAR-3 human ovarian cancer cells. MTT viability, BrdU and cell counting assays were used to study the cell proliferation effect of adenosine in presence of adenosine deaminase inhibitor and the nucleoside transporter inhibitor. Cell cycle analysis, propidium iodide and annexin V staining, caspase-3 activity assay, cyclinD1, Cdk4, Bcl-2 and Bax protein expressions were assessed to detect apoptosis. Adenosine significantly inhibited cell proliferation in a concentration-dependent manner in OVCAR-3 cell line. Adenosine induced cell cycle arrest in G0/G1 phase via Cdk4/cyclinD1-mediated pathway. Adenosine induced apoptosis, which was determined by Annexin V-FITC staining and increased sub-G1 population. Moreover, down-regulation of Bcl-2 protein expression, up-regulation of Bax protein expression and activation of caspase-3 were observed in response to adenosine treatment. The results of this study suggest that extracellular adenosine induced G1 cell cycle arrest and apoptosis in ovarian cancer cells via cyclinD1/ Cdk4 and Bcl-2/Bax pathways and caspase-3 activation. These data might suggest that adenosine could be used as an agent for the treatment of ovarian cancer.
File Format	HTM / HTML
ISSN	10104283
Issue Number	2
Volume Number	34
e-ISSN	14230380
Journal	Tumor Biology
Language	English
Publisher	Springer
Publisher Date	2013-04-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Medicine__semicolon__oncology Adenosine Pharmacology Apoptosis Drug Effects Cell Cycle Checkpoints Cyclin D1 Metabolism Cyclin-dependent Kinase 4 Ovarian Neoplasms Pathology Proto-oncogene Proteins C-bcl-2 Bcl-2-associated X Protein Blotting, Western Cell Cycle Cell Proliferation Female Flow Cytometry Humans Drug Therapy Tumor Cells, Cultured Vasodilator Agents Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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