NDLI: BAG3 regulates cell proliferation, migration, and invasion in human colorectal cancer.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Shi, Huiyong Xu, Haidong Li, Zengjun Zhen, Yanan Wang, Bin Huo, Shoujun Xiao, Ruixue Xu, Zhongfa
Description	Country affiliation: China Author Affiliation: Shi H ( Department of General Surgery, Affiliated Hospital of Shandong Academy of Medical Sciences, 38# wuyingshan Road, Jinan, Shandong, China.); Xu H ( Department of General Surgery, Affiliated Hospital of Shandong Academy of Medical Sciences, 38# wuyingshan Road, Jinan, Shandong, China.); Li Z ( Department of General Surgery, Shandong Cancer Hospital and Institute, Jinan, Shandong, China.); Zhen Y ( Department of General Surgery, Affiliated Hospital of Shandong Academy of Medical Sciences, 38# wuyingshan Road, Jinan, Shandong, China.); Wang B ( Department of General Surgery, Affiliated Hospital of Shandong Academy of Medical Sciences, 38# wuyingshan Road, Jinan, Shandong, China.); Huo S ( Department of General Surgery, Affiliated Hospital of Shandong Academy of Medical Sciences, 38# wuyingshan Road, Jinan, Shandong, China.); Xiao R ( Department of General Surgery, Affiliated Hospital of Shandong Academy of Medical Sciences, 38# wuyingshan Road, Jinan, Shandong, China.); Xu Z ( Department of General Surgery, Affiliated Hospital of Shandong Academy of Medical Sciences, 38# wuyingshan Road, Jinan, Shandong, China. xuzfshandong@163.com.)
Abstract	Bcl2-associated athanogene 3 (BAG3) has been reported to be elevated in various tumors. However, it is unclear whether BAG3 has a functional role in the initiation and progression of colorectal cancer (CRC). Here, we collected CRC samples and cell lines to validate the pathway by using gene and protein assays. RT-PCR showed that the expression of BAG3 mRNA in CRC tissues was obviously higher than that in non-tumor tissues (p < 0.001). Immunohistochemical analysis showed that immunoreactivity of BAG3 was found in most CRC tissues and strongly correlated with TNM stage (p = 0.001), differentiation (p = 0.003), and metastasis (p = 0.010). Low expression of BAG3 in HCT-8 significantly reduced cellular proliferation, migration, and invasion. The analysis of in vitro cell showed that HCT-8 cells were exposed to si-BAG3, and its growth was inhibited depending on modulation of cell cycle G1/S checkpoints and cell cycle regulators, involving cyclin D1, cyclin A2, and cyclin B1. Furthermore, suppression of the epithelial-mesenchymal transition (EMT) by si-BAG3 is linked to the decreased expression of E-cadherin and the increased expression of N-cadherin, vimentin, and MMP9. In conclusion, in the present study, we demonstrated that BAG3 overexpression plays a critical role in cell proliferation, migration, and invasion of colorectal cancer. Our data suggests targeted inhibition of BAG3 may be useful for patients with CRC.
File Format	HTM / HTML
ISSN	10104283
Issue Number	4
Journal	Tumor Biology
Volume Number	37
e-ISSN	14230380
Language	English
Publisher	Springer
Publisher Date	2016-04-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in