NDLI: Emerging prognostic markers related to mesenchymal characteristics of poorly differentiated breast cancers.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Scimeca, Manuel Antonacci, Chiara Colombo, Daniele Bonfiglio, Rita Buonomo, Oreste Claudio Bonanno, Elena
Description	Country affiliation: Italy Author Affiliation: Scimeca M ( Anatomic Pathology Section, Department of Biomedicine and Prevention, University of Rome 'Tor Vergata', Via Montpellier 1, 00133, Rome, Italy.); Antonacci C ( TMALab s.r.l., Spin-off of University of Tor Vergata, Rome, Italy.); Colombo D ( Anatomic Pathology Section, Department of Biomedicine and Prevention, University of Rome 'Tor Vergata', Via Montpellier 1, 00133, Rome, Italy.); Bonfiglio R ( TMALab s.r.l., Spin-off of University of Tor Vergata, Rome, Italy.); Buonomo OC ( Anatomic Pathology Section, Department of Biomedicine and Prevention, University of Rome 'Tor Vergata', Via Montpellier 1, 00133, Rome, Italy.); Bonanno E ( Anatomic Pathology Section, Department of Biomedicine and Prevention, University of Rome 'Tor Vergata', Via Montpellier 1, 00133, Rome, Italy.)
Abstract	Despite the screening program, breast cancer is the commonest cause of cancer death in women in the industrialized world. In this study, we investigate the correlation among poorly differentiated carcinoma, epithelial to mesenchymal transition (EMT) phenomenon, and expression of NF-kB, Sonic Hedgehog (SHH), K-RAS, and PTX3 in breast cancer in 100 breast biopsies. Samples were classified as follows: 30 benign lesions (BL), 30 ductal infiltrating carcinomas low grade (MLG1), and 40 ductal infiltrating carcinomas high grade (MLG3). Expression of vimentin, CD44, ß-catenin, NF-kB, SHH, K-RAS, CD44, and PTX3 was studied by immunohistochemistry. The different rate of cells with vimentin, nuclear ß-catenin, and CD44 expression in MLG3 as compared with MLG1 and BL suggested that the process of de-differentiation of breast cancer cells could be related to the EMT. Our results showed a significant increase in NF-kB signal in MLG3 (2.33 ± 0.77) with respect to MLG1 (1.26 ± 0.55) and BL (0.86 ± 0.52). SHH expression appeared low in BL (1.00 ± 0.41) and homogenously widespread in MLG1 (1.23 ± 0.63) and MLG3 (1.56 ± 0.54). An important increase in K-RAS signal was observed in MLG3 compared to that in BL (2.20 ± 0.69 vs 0.82 ± 0.59). As regards PTX3, we observed a strong expression in MLG3 (2.00 ± 0.78) with respect to BL (0.58 ± 0.55) and MLG1 (1.53 ± 0.76). The recurring expression of NF-kB, SHH, K-RAS, and PTX3 in vimentin- and CD44-positive breast cancer cells allows to speculate that breast cells acquire the ability to express these molecules in concomitance to EMT phenomenon.
File Format	HTM / HTML
ISSN	10104283
Issue Number	4
Journal	Tumor Biology
Volume Number	37
e-ISSN	14230380
Language	English
Publisher	Springer
Publisher Date	2016-04-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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