NDLI: Tagging staphylococcal enterotoxin B (SEB) with TGFaL3 for breast cancer therapy.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yousefi, Forough Siadat, Seyed Davar Saraji, Alireza Azizi Hesaraki, Saeed Aslani, Mohammad Mehdi Mousavi, Seyed Fazlollah Imani Fooladi, Abbas Ali
Description	Country affiliation: Iran Author Affiliation: Yousefi F ( Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.); Siadat SD ( Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.); Saraji AA ( Department of Medical Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.); Hesaraki S ( Department of Pathobiology, Science and Research Branch, Islamic Azad University, Tehran, Iran.); Aslani MM ( Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran.); Mousavi SF ( Department of Bacteriology, Pasteur Institute of Iran, Tehran, Iran. mousavi@pasteur.ac.ir.); Imani Fooladi AA ( Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran. imanifouladi.a@gmail.com.)
Abstract	Recent research has attempted to direct superantigens towards tumors by means of tumor-targeted superantigen (TTS) strategy. In this study, we explored the antitumor property of TTS by fusing the third loop of transforming growth factor (TGF L3) to staphylococcal enterotoxin type B (SEB) and investigated the possibility of the therapeutic application of TGF L3-SEB as a novel antitumor candidate in mice bearing breast cancer. Treatment was performed through intratumoral and intravenous injection of TGF L3-SEB. Tumor size/volume, long-term survival, and cytokine secretion were assessed. In addition, the toxicity of each treatment on liver and kidneys was examined. Our results indicated that the relative tumor volume significantly increased in the mice receiving intratumoral TGFaL3-SEB (p < 0.05). Surprisingly, 5 out of the 14 mice were cleared from the tumor thoroughly in 10-25 days after intratumoral administration of TGFaL3-SEB. Quantification of cytokines clearly showed that the mice receiving intratumoral SEB significantly secreted higher interferon Î³ (IFN-Î³) and tumor necrosis factor (TNF- ) compared with the other groups (p < 0.05). The antitumor effect was followed by inhibition of cell proliferation (Ki-67) and micro vascularization (CD31). The highest and lowest levels of tumor necrosis were observed in the intratumoral administration of TGF L3-SEB (85 %) and PBS (14 %), respectively. Intratumoral injection of TGF L3-SEB increased the lifespan of the mice so 37.5 % of them could survive for more than 6 months (p < 0.05). Overall, our findings indicated that intratumoral administration of TGF L3-SEB effectively inhibited the growth of breast tumors through induction of necrosis and suppressing proliferation and angiogenesis without systemic toxicity.
File Format	HTM / HTML
ISSN	10104283
Issue Number	4
Journal	Tumor Biology
Volume Number	37
e-ISSN	14230380
Language	English
Publisher	Springer
Publisher Date	2016-04-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in