| Author |
Zheng, Wei Zhao, Ting Feng, Weiwei Wang, Wei Zou, Ye Zheng, Daheng Takase, Mohammed Li, Qian Wu, Huiyu Yang, Liuqing Wu, Xiangyang |
| Description |
Country affiliation: China Author Affiliation: Zheng W ( School of Pharmacy, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, Jiangsu, China.); Zhao T ( School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.); Feng W ( School of Food and Biological Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.); Wang W ( School of Food and Biological Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.); Zou Y ( School of Food and Biological Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.); Zheng D ( School of Food and Biological Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.); Takase M ( School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China.); Li Q ( School of Pharmacy, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, Jiangsu, China.); Wu H ( School of Pharmacy, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, Jiangsu, China.); Yang L ( School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, China. Electronic address: yangliuqing@ujs.edu.cn.); Wu X ( School of the Environment, Jiangsu University, Xuefu Rd. 301, Zhenjiang 212013, Jiangsu, China. Electronic address: wuxy@ujs.edu.cn.) |
| Abstract |
A water-soluble polysaccharide (OFPS11) was obtained from okra (Abelmoschus esculentus) flowers using aqueous extraction and purification with DEAE-52 cellulose and Sephacryl™ S-500 column. Its preliminary characterization and immunomodulating activity were investigated. Results showed that OFPS11 is mainly composed of galactose and rhamnose in a molar ratio of 2.23:1 with molecular mass of 1,700 kDa. RAW264.7 cells pretreated with OFPS11 significantly inhibited the proliferation of HepG-2 cells. Additionally, OFPS11 enhanced the phagocytic ability and induced the elevation of NO production, TNF- and IL-1ß secretion of RAW264.7 cells. Furthermore, OFPS11 promoted both the expression of iNOS protein and of iNOS and TNF- mRNA. OFPS11 can strongly increase NF-κB levels in nucleuses, which is an important transcription factor that can modulate expressions of iNOS, NO and TNF- . These outcomes support that OFPS11 exerts its antitumor activity by probably stimulating macrophage activities through nuclear NF-κB pathway. |
| Subject Keyword |
Discipline Biochemistry__semicolon__materials Discipline Science Abelmoschus Chemistry Immunologic Factors Isolation & Purification Polysaccharides Animals Antineoplastic Agents, Phytogenic Pharmacology Cell Line Cell Nucleus Metabolism Cytotoxicity, Immunologic Drug Effects Drug Evaluation, Preclinical Flowers Hep G2 Cells Humans Macrophages Mice Molecular Weight Nf-kappa B Nitric Oxide Biosynthesis Nitric Oxide Synthase Type Ii Genetics Phagocytosis Rna, Messenger Solubility Transcription, Genetic Tumor Necrosis Factor-alpha Up-regulation Water Journal Article Research Support, Non-u.s. Gov't |
