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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kazlowski, Bartosz Ko, Yuan-Tih |
| Description | Author Affiliation: Kazlowski B ( Department of Food Science, Biotechnology Division, College of Life Sciences, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung, 20224, Taiwan, ROC. Electronic address: bart_kazlowski@hotmail.com.); Ko YT ( Department of Food Science, Biotechnology Division, College of Life Sciences, National Taiwan Ocean University, 2 Pei-Ning Road, Keelung, 20224, Taiwan, ROC. Electronic address: irisko@ntou.edu.tw.) |
| Abstract | A series of linear glucan saccharides (GS) with defined quantity and degree of polymerization (DP) were synthesized from -d-glucose 1-phosphate ( -d-Glc 1-P) by phosphorylase-a. The GS product fractions with average DP 11, 22, 38, 52, 60, 70, and 79 were measured by HPSEC-ELSD system. Then the same seven fractions were resolved into individual peaks with DP: 6-14, 10-32, 27-55, 37-67, 44-75, 49-83 and 53-89 by HPAEC-PAD system. Results showed that measurement of -d-Glc 1-P amount consuming during GS synthesis by both systems enable calculation of reaction yield. The reaction yield for the 24h biosynthesis of the GS product was 25.3% (measured by HPSEC-ELSD) or 29.1% (measured by HPAEC-PAD). The HPSEC-ELSD and HPAEC-PAD systems were also successfully used for phosphorylase-a activity measurement in order to perform its kinetic characterization. This study established feasible systems for preparation of various sizes of the GS with defined DP and quantity as well as characterization of phosphorylase-a kinetics. |
| File Format | HTM / HTML |
| ISSN | 01448617 |
| Volume Number | 106 |
| e-ISSN | 18791344 |
| Journal | Carbohydrate Polymers |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-06-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry__semicolon__materials Discipline Science Glucans Chemical Synthesis Glucosephosphates Chemistry Phosphorylase a Polymers Polysaccharides Animals Calibration Chromatography, Gel Methods Chromatography, High Pressure Liquid Chromatography, Ion Exchange Isolation & Purification Nuclear Magnetic Resonance, Biomolecular Polymerization Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Materials Chemistry Polymers and Plastics |
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