NDLI: The effect of bovine rotavirus and its nonstructural protein 4 on ER stress-mediated apoptosis in HeLa and HT-29 cells

Content Provider	Springer Nature Link
Author	Goodarzi, Zahra Soleimanjahi, Hoorieh Arefian, Ehsan Saberfar, Esmaeil
Copyright Year	2015
Abstract	Endoplasmic reticulum (ER) plays important roles in multiple cellular processes as well as cell survival and apoptosis. Perturbation of ER functions leads to ER stress and unfolded protein response (UPR). The primary goal of this response is cell survival, but severe ER stress can trigger apoptosis signaling. In tumor cells, chronically activated UPR response provides tumor growth. So, apoptosis induced by the ER stress has been the target for anti-cancer therapy. In this in vitro study, we examined the apoptotic effect associated with ER stress of bovine rotavirus and its nonstructural protein 4 (NSP4) alone in two cancer cell lines. The plasmid pcDNA3.1 encoding NSP4 protein of bovine rotavirus transfected with lipofectamine 2000 into the HeLa and HT-29 cells for protein production. MTT, flow cytometry, and Western blot were used to evaluate the cell viability, apoptosis, and expression level of C/EBP-homologous protein (CHOP) and activated caspase-4. In parallel, the apoptotic effect of the bovine rotavirus associated with ER stress in the infected cells was examined too. The cytotoxic and apoptotic effect of NSP4 protein on the cells were statistically significant compared to the control groups. However, Western blot showed that the expression of the NSP4 protein by recombinant plasmid did not lead to high expression of CHOP and activation of caspase-4. Interestingly, rotavirus not only induced significant apoptosis but also caused an increase in CHOP expression and caspase-4 activation in the infected cells compared to control. As a result, NSP4 protein and bovine rotavirus can be considered a potential novel bio-therapeutic strategy for cancer treatment.
Starting Page	3155
Ending Page	3161
Page Count	7
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	37
Issue Number	3
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-10-01
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Apoptosis CHOP Caspase-4 Endoplasmic reticulum ER stress Rotavirus Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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