NDLI: Expression of CDC5L is associated with tumor progression in gliomas

Content Provider	Springer Nature Link
Author	Chen, Wenjuan Zhang, Li Wang, Yan Sun, Jie Wang, Donglin Fan, Shaochen Ban, Na Zhu, Junya Ji, Bin Wang, Yuchan
Copyright Year	2015
Abstract	Cell division cycle 5-like (CDC5L) protein is a cell cycle regulator of the G$_{2}$/M transition and has been reported to participate in the catalytic step of pre-messenger RNA (mRNA) splicing and DNA damage repair. Recently, it was also found to act as a candidate oncogene in osteosarcoma and cervical tumors. However, the role of CDC5L expression in tumor biology was still unclear. Here, we analyzed the expression and clinical significance of CDC5L in gliomas. The expression of CDC5L in fresh glioma tissues and paraffin-embedded slices was evaluated by western blot and immunohistochemistry, respectively. We found that CDC5L was highly expressed in glioma tissues. The expression of CDC5L was significantly associated with glioma pathology grade and Ki-67 expression. Univariate and multivariate analyses showed that high CDC5L expression was an independent prognostic factor for glioma patients’ survival. To determine whether CDC5L could regulate the proliferation of glioma cells, we transfected glioma cells with interfering RNA target CDC5L, then investigated cell proliferation with cell counting kit (CCK)-8, flow cytometry assays and colony formation analyses. Our results indicated that knockdown of CDC5L would inhibit proliferation of glioma cells. Besides, reduced expression of CDC5L could induce the apoptosis of glioma cells. These findings suggested that CDC5L might play an important role in glioma and thus be a promising therapeutic target of glioma.
Starting Page	4093
Ending Page	4103
Page Count	11
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	37
Issue Number	3
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-10-21
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	CDC5L Glioma Proliferation Apoptosis Prognosis Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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