NDLI: Association of cytoplasmic p27 expression with an unfavorable response to cisplatin-based chemotherapy and poor outcomes in non-small cell lung cancer

Content Provider	Springer Nature Link
Author	Lin, Tsang Chi Tsai, Lung Hung Chou, Ming Chih Chen, Chih Yi Lee, Huei
Copyright Year	2015
Abstract	Reduced nuclear p27 expression is associated with a poor outcome in various cancers, including non-small cell lung cancer (NSCLC). Cytoplasmic p27 expression was shown to be associated with an unfavorable response to chemotherapy and poor outcomes in some carcinomas, but it has not been well studied in NSCLC. Herein, p27 expression in 219 tumors surgically resected from NSCLC patients was evaluated by immunohistochemistry (IHC). The most common of p27 immunostaining in lung tumors was observed in the cytoplasm (N−/C+, 32 %), followed by negative (N−/C−, 29 %), nucleus (N+/C−, 24 %), and nucleus plus cytoplasm (N+/C+, 15 %). Kaplan–Meier and Cox regression models showed that p27 N−/C+ tumors exhibited the worst overall survival (OS) and relapse-free survival (RFS) among the four categories of tumors. Among 135 of 219 patients who received cisplatin-based chemotherapy, p27 N−/C+ tumors most commonly showed an unfavorable response to cisplatin-based chemotherapy, followed by p27 N−/C− tumors when p27 N+/C− tumors were used as a reference. IHC analysis for phosphorylated extracellular signal-regulated kinase (p-ERK) and Bcl-2 expression in the lung tumors was performed to test whether ERK activation could enhance p27 nuclear export and the expression of Bcl-2 to test whether ERK activation could enhance p27 nuclear export and Bcl-2 expression. The data showed that p-ERK expression was positively correlated with cytoplasmic p27 (N−/C+) and Bcl-2 expression in the lung tumors. Patients with high Bcl-2-expressing tumors treated with cisplatin-based chemotherapy showed unfavorable predictive values in a subset of this study population. Therefore, we suggest that cytoplasmic p27 (N−/C+) via ERK-activated Bcl-2 expression may predict an unfavorable response to cisplatin-based chemotherapy and poor outcomes in NSCLC.
Starting Page	4017
Ending Page	4023
Page Count	7
File Format	PDF
ISSN	10104283
Journal	Tumor Biology
Volume Number	37
Issue Number	3
e-ISSN	14230380
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-10-20
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	p27 Cisplatin-based chemotherapy Non-small cell lung cancer Cancer Research
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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