Loading...
Please wait, while we are loading the content...
Similar Documents
Determination of sertraline in human plasma by high-performance liquid chromatography-electrospray ionization mass spectrometry and method validation.
| Content Provider | Semantic Scholar |
|---|---|
| Author | He, Lijuan Feng, Fang |
| Copyright Year | 2005 |
| Abstract | A sensitive, simple, and specific liquid chromatographic method coupled with electrospray ionization-mass spectrometry (MS) is presented for the determination of sertraline in plasma. With zaleplon as the internal standard, sertraline is extracted from the alkalized plasma with cyclohexane. The organic layer is evaporated and the residue is redissolved in the mobile phase of methanol-10 mmol/L ammonium acetate solution-acetonitrile (62:28:10, v/v/v). An aliquot of 20 microL is chromatographically analyzed on a Shimadzu ODS C18 column (5 microm, 150- x 4.6-mm i.d.) by means of selected-ion monitoring mode of MS. The calibration curve of sertraline in plasma exhibits a linear range from 0.5 to 25.0 ng/mL with a correlation coefficient of 0.9998. The limit of quantitation is 0.5 ng/mL. The intra- and interday variations (relative standard deviation) are less than 7.8% and 9.5% (n = 5), respectively. The application of this method is demonstrated for the analysis of sertraline plasma samples in a human pharmacokinetic study. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | https://oup.silverchair-cdn.com/oup/backfile/Content_public/Journal/chromsci/43/10/10.1093/chromsci/43.10.532/2/43-10-532.pdf?Expires=1492980219&Key-Pair-Id=APKAIUCZBIA4LVPAVW3Q&Signature=Va~r~DE774v8uFxYik~yqpuxHkMX2Nrt9WFwp6UhhgN9-Tl1mxac0tvbAEjYsC9XKoCsx0VKa0V2fhDJY5X-8SMMAJWCLNMQynEgDULJgo9nHfmOf1Sj0~uvFRTG--q6q29gRdGdyRym8gR3jCtnJ4gpO1zCf4qU9-JA5GOBu2C7Tl-ZLLaxqeCqu2e5t7SS733-dv1cz3xC1kTTZxmWriVyPPIlTSa8fh8UjtGGkG74A3Ww7CkcDr0KbN067at0Bc6RAPaj5yMJZbHhkftKc6NzEysnABqQtcUjBDi6Q1zHIP26kgSmJyrEZGbZ05Jp5R9li-jNoMeaHADovRGMmQ__ |
| PubMed reference number | 16438795v1 |
| Volume Number | 43 |
| Issue Number | 10 |
| Journal | Journal of chromatographic science |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Aliquotting Coefficient Cyclohexane Eluent Exhibits as Topic Extraction Ions Liquid Chromatography Mass Spectrometry Quantitation Sertraline Spectrometry, Mass, Electrospray Ionization Standard deviation Volume fraction acetonitrile ammonium acetate zaleplon |
| Content Type | Text |
| Resource Type | Article |
