NDLI: Haemolysis effect of Mefenamic Acid 250 mg Capsule in Bio analysis by liquid chromatography-tandem mass spectrometry

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Haemolysis effect of Mefenamic Acid 250 mg Capsule in Bio analysis by liquid chromatography-tandem mass spectrometry

Content Provider	Semantic Scholar
Author	Mistry, Paresh Menon, Shobhna
Copyright Year	2013
Abstract	A rapid, simple and specific method for estimation of Mefenamic acid in human plasma was validated using Indomethacin as internal standard. The analyte and internal standard were extracted from plasma using simple solid phase extraction. The compound were separated on a reverse-phase column with an isocratic mobile phase consisting of 2 mM Ammonium Acetate in Water and acetonitrile (20:80, v/v) and detected by tandem mass spectrometry in negative ion mode. The ion transition recorded in multiple reaction monitoring mode were m/z 240.1 196.0 for Mefenamic acid and m/z 356.1312.0 for internal standard. Linearity in plasma was observed over the concentration range 35.000 – 7000.000 ng/mL for Mefenamic acid. The cv of the assay was 4.89 % to 5.98 % and accuracy was 99.36 to 102.20 % Intra and Interday respectively at LLOQ level. The validated method was applied to bioequivalence study of 250 mg Mefenamic acid in 28 healthy human volunteers. Total 50 samples from individual volunteers identified as Haemolyzed which were analyze initial and repeat again to cross check the method reproducibity for Haeamolysis effect and compared which found acceptable range.
File Format	PDF HTM / HTML
Alternate Webpage(s)	http://www.iosrjournals.org/iosr-jac/papers/vol5-issue5/D0551724.pdf?id=8022
Language	English
Access Restriction	Open
Subject Keyword	Eluent Emoticon Extraction Hemolysis (disorder) Indomethacin Ions Liquid Chromatography Lower Limit of Quantitation Mefenamic Acid Plasma Active Tandem Mass Spectrometry Two-phase locking Virtual 8086 mode Volume fraction acetonitrile ammonium acetate analyte
Content Type	Text
Resource Type	Article

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