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Determination and pharmacokinetics of manidipine in human plasma by HPLC/ESIMS.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Jing, Jing Ren, Weichao Chen, Xi-Jing Zhou, Wei Zhu, Xuping Sun, Yuegao Wang, Guang-Ji |
| Copyright Year | 2007 |
| Abstract | A sensitive HPLC/ESIMS method was established for the determination of manidipine in human plasma and pharmacokinetics study. After basified plasma with ammonia, manidipine and the internal standard (IS) (felodipine) were extracted with n-hexane and separated on a Hypersil ODS2 column with a mobile phase of methanol-5 mm ammonium acetate solution containing 0.1% acetic acid (85:15, v/v). MS determination was performed by electrospray ionization in the selected ion monitoring mode. Manidipine was monitored at m/z 611.4 and IS at m/z 384. The assay had a calibration range from 0.2 to 20 ng/mL and a lower limit of quantification of 0.1 ng/mL. The method has been successfully applied to the pharmacokinetic study in healthy volunteers. |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://www.dmpkd.com/doc/2007paper03.pdf |
| PubMed reference number | 17428020v1 |
| Volume Number | 21 |
| Issue Number | 8 |
| Journal | Biomedical chromatography : BMC |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Acetic Acid Ammonia measurement Calibration Eluent Extraction Felodipine Ions Quantitation Spectrometry, Mass, Electrospray Ionization Volume fraction ammonium acetate manidipine medroxyprogesterone acetate n-hexane |
| Content Type | Text |
| Resource Type | Article |
