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Simultaneous determination of benazepril hydrochloride and benazeprilat in plasma by high-performance liquid chromatography/electrospray-mass spectrometry.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Xiao, Weitao Chen, Bo Yao, Shouzhuo Cheng, Zeneng |
| Copyright Year | 2005 |
| Abstract | An analytical method for simultaneous determination of benazepril and its active metabolite, benazeprilat, in human plasma by high-performance liquid chromatography/electrospray-mass spectrometry was developed and validated. Rutaecarpine was selected as the internal standard. The separation was achieved on a C(18) column with acetonitrile and aqueous solution (0.1% formic acid) as mobile phase with a gradient mode. The quantification of target compounds was using a selective ionization recording at m/z 425.5 for benazepril, m/z 397.5 for benzeprilat and m/z 288.3 for rutaecarpine. The correlation coefficients of the calibration curves were better than 0.992 (n = 6), in the range of 6.67-666.67 ng/ml for benazepril and benazeprilat. The inter- and intra-day accuracy, precision, linear range had been investigated in detail. The method can be used to assess the bioavailability and pharmacokinetics of the drug. |
| Starting Page | 53 |
| Ending Page | 56 |
| Page Count | 4 |
| File Format | PDF HTM / HTML |
| Alternate Webpage(s) | http://hgxy.hunnu.edu.cn/UPwork/2006111314285885599.pdf |
| PubMed reference number | 15639452v1 |
| Volume Number | 814 |
| Issue Number | 2 |
| Journal | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
| Language | English |
| Access Restriction | Open |
| Subject Keyword | Benazepril hydrochloride Calibration Eluent Gradient Liquid Chromatography Quantitation Spectrometry acetonitrile benazeprilat formic acid rutecarpine |
| Content Type | Text |
| Resource Type | Article |
