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Analysis of free ACh and 5-HT in milk from four different species and their bioactivity on 5-HT₃ and nACh receptors

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Food & Function Vol - 5 Issue - 6

Food & Function Vol - 5 Issue - 5

Food & Function Vol - 5 Issue - 4

Food & Function Vol - 5 Issue - 3

Food & Function Vol - 5 Issue - 2

Food & Function Vol -4

Food & Function Vol -3

Food & Function Vol -2

Food & Function Vol -1

Analysis of free ACh and 5-HT in milk from four different species and their bioactivity on 5-HT₃ and nACh receptors

Content Provider	Royal Society of Chemistry (RSC)
Author	Miledi, Ricardo Limon, Agenor Reyes-Ruiz, Jorge M. Alshanqeeti, Ali S. Gallegos-Perez, Jose-Luis Aljohi, Mohammad A.
Copyright Year	2014
Abstract	Milk is one of the most beneficial aliments and is highly recommended in normal conditions; however, in certain disorders, like irritable bowel syndrome, cow milk and dairy products worsen the gastric symptoms and their use is not recommended. Among the most recognized milk-induced gatrointestinal symptoms are abdominal pain, nausea and vomiting, which are processes controlled by cholinergic and serotonergic transmission. Whether the presence of bioavailable ACh and 5-HT in milk may contribute to normal peristalsis, or to the developing of these symptoms, is not known. In this work we attempt to determine whether the content of free ACh and 5-HT is of physiological significance in milk from four different species: cow (bovine), goat, camel and human. Liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) was used to identify and quantify free ACh and 5-HT in milk, and activation of the serotonergic and cholinergic ionotropic receptors was investigated using electrophysiological experiments. Our principal hypothesis was that milk from these four species had sufficient free ACh and 5-HT to activate their correspondent receptors expressed in a heterologous system. Our results showed a more complex picture, in which free ACh and 5-HT and their ability to activate cholinergic and serotonergic receptors are not correlated. This work is a first step to elucidate whether 5-HT and ACh, at the concentrations present in the milk, can be associated to a direct function in the GI.
Starting Page	1489
Ending Page	1494
Page Count	6
File Format	HTM / HTML PDF
ISSN	20426496
Volume Number	5
Issue Number	7
Journal	Food & Function
DOI	10.1039/c3fo60458f
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Irritable bowel syndrome Syndrome Nausea Cholinergic Serotonin Peristalsis Goat Camel Chromatography Ligand-gated ion channel 5-HT receptor
Content Type	Text
Resource Type	Article
Subject	Medicine Food Science

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