NDLI: Short-Term Regulation of Murine Colonic NBCe1-B (Electrogenic Na+/HCO3 − Cotransporter) Membrane Expression and Activity by Protein Kinase C

Content Provider	PubMed Central
Author	May, Oliver Yu, Haoyang Riederer, Brigitte Manns, Michael P. Seidler, Ursula Bachmann, Oliver
Editor	Kanzaki, Makoto
Copyright Year	2014
Abstract	The colonic mucosa actively secretes HCO3 −, and several lines of evidence point to an important role of Na+/HCO3 − cotransport (NBC) as a basolateral HCO3 − import pathway. We could recently demonstrate that the predominant NBC isoform in murine colonic crypts is electrogenic NBCe1-B, and that secretagogues cause NBCe1 exocytosis, which likely represents a component of NBC activation. Since protein kinase C (PKC) plays a key role in the regulation of ion transport by trafficking events, we asked whether it is also involved in the observed NBC activity increase. Crypts were isolated from murine proximal colon to assess PKC activation as well as NBC function and membrane abundance using fluorometric pHi measurements and cell surface biotinylation, respectively. PKC isoform translocation and phosphorylation occurred in response to PMA-, as well as secretagogue stimulation. The conventional and novel PKC inhibitors Gö6976 or Gö6850 did not alter NBC function or surface expression by themselves, but stimulation with forskolin (10−5 M) or carbachol (10−4 M) in their presence led to a significant decrease in NBC-mediated proton flux, and biotinylated NBCe1. Our data thus indicate that secretagogues lead to PKC translocation and phosphorylation in murine colonic crypts, and that PKC is necessary for the increase in NBC transport rate and membrane abundance caused by cholinergic and cAMP-dependent stimuli.
Related Links	http://dx.doi.org/10.1371/journal.pone.0092275
Starting Page	92275
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	3
Volume Number	9
Language	English
Publisher	Public Library of Science
Publisher Date	2014-03-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Short-Term Regulation of Murine Colonic NBCe1-B (Electrogenic Na+/HCO3 − Cotransporter) Membrane Expression and Activity by Protein Kinase C

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