Please wait, while we are loading the content...
Please wait, while we are loading the content...
| Content Provider | IET Digital Library |
|---|---|
| Author | Zhang, Chun ping Chang, Yaw Jen Ding, Zi Huan Chang, Cheng Hao |
| Abstract | This Letter describes the implementation of a droplet-based protein chip with an Ni–Co alloy layer for conducting immunoassays. The proposed chip consists of a substrate of a printed circuit board and a microchannel layer. On the substrate surface, an Ni–Co coating was fabricated by electrodeposition to immobilise the histidine-tagged protein. Through droplet fusion and mixing, the fused droplets aligned at the downstream position of the microchannel and were immobilised by the Ni–Co layer for further incubation and fluorescence detection. The experimental results showed that the sample which is encapsulated by the carrier fluid does not have evaporation and contamination problems. Therefore strong fluorescence intensity proportional to the sample concentration can be obtained. The fluorescence intensity is also related to the droplet size. An extremely low concentration of encapsulated content in a smaller droplet might result in arduous fluorescence detection. This proposed droplet-based protein chip allows the operation sequence of the immunoassay to be conducted automatically through the manipulation of droplets. Moreover, the fabrication process is simple and the chip can be implemented to immobilise any functional proteins with His-tag attached. |
| Starting Page | 718 |
| Ending Page | 721 |
| Page Count | 4 |
| Volume Number | 8 |
| e-ISSN | 17500443 |
| Issue Number | Issue 10, Oct (2013) |
| Alternate Webpage(s) | https://digital-library.theiet.org/content/journals/mnl/8/10 |
| Alternate Webpage(s) | https://digital-library.theiet.org/content/journals/10.1049/mnl.2013.0376 |
| Journal | Micro & Nano Letters |
| Access Restriction | Open |
| Rights Holder | © The Institution of Engineering and Technology |
| Subject Keyword | Applied Fluid Mechanics Biochemistry BioMEMS Biophysical Instrumentation And Technique Chemical Analysis And Related Physical Method of Analysis Cobalt Alloys Droplet Fusion Droplet Size Droplet-based Protein Chip Drops Electrodeposition Fluorescence Fluorescence Intensity Histidine-tagged Protein Immobilisation Immunoassays Incubation Lab-on-a-chip MEMS And NEMS Device Technology Microchannel Layer Microfluidics Micromechanical And Nanomechanical Device And System Mixing Nickel Alloy NiCo Ni–Co Coated Surface Printed Circuit Board Substrate Proteins |
| Content Type | Text |
| Resource Type | Article |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
| Sl. | Authority | Responsibilities | Communication Details |
|---|---|---|---|
| 1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
| 2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
| 3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
| 4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
| 5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
| 6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
| 7 | National Digital Library of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
| 8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
| 9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
|
Loading...
|