NDLI: Fluorescence Lifetime Imaging of Haematoxylin and Eosin-stained Cervical Tissue

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2009 International Conference on Biomedical and Pharmaceutical Engineering

2006 International Conference on Biomedical and Pharmaceutical Engineering

2006 International Conference on Biomedical and Pharmaceutical Engineering

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Fluorescence Lifetime Imaging of Haematoxylin and Eosin-stained Cervical Tissue

Content Provider	IEEE Xplore Digital Library
Author	Fu, C.Y. Dinish, U.S. Ng, B.K. Murukeshan, V.M. Seah, L.K. Lim-Tan, S.K.
Copyright Year	2006
Description	Author affiliation: Nanyang Technol. Univ., Nanyang (Fu, C.Y.)
Abstract	This article presents the fluorescence lifetime characterization of haematoxylin and eosin (H&E)-stained cervical tissue using a wide-field time-domain fluorescence lifetime imaging microscope (FLIM). The transient response of epithelial fluorescence was investigated and approximated using a bi-exponential model. It was found that the fluorescence lifetimes of eosin can be correlated to the tissue histology. The preliminary result suggests that the tumor-associated molecules are retained in the tissues even after tissue fixation and staining. The developed FLIM system was successfully applied to detect the histological changes in the tissue samples.
Starting Page	368
Ending Page	372
File Size	417004
Page Count	5
File Format	PDF
ISBN	9789810579
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2006-12-11
Publisher Place	Singapore
Access Restriction	Subscribed
Rights Holder	NTU
Subject Keyword	Optical filters Transient response Hospitals Microscopy Wavelength measurement Fluorescence Optical imaging Cameras Cervical cancer Neoplasms
Content Type	Text
Resource Type	Article

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