NDLI: Porcine Small Intestinal Submucosa is a Suitable Small Diameter Vascular Substitute

Content Provider	IEEE Xplore Digital Library
Author	Toy, W. Gan, Y. Law, S. Jh Yeo Chiu, J.J. Lj Chen Hwang, H.C.
Copyright Year	2006
Description	Author affiliation: Nat. Inst. of Educ., Singapore (Toy, W.; Gan, Y.)
Abstract	Biomaterials have become the critical component in the development of small diameter vascular graft. In this project, the suitability of a biomaterial, rich in collagen and other growth factors, derived from the porcine small intestine submucosa (SIS) to be used as a small diameter vascular substitute was studied. Endothelial cells (ECs) are the innermost cell linings of the lumen of all blood vessels. The morphology of ECs cultured on SIS was examined in vitro using scanning electron microscopy. Results showed that endothelial cells became round shape, proliferated and attached to the surface of SIS to form a confluent monolayer. Organization of the cytoskeleton of the endothelial cells cultured on SIS and on glass slides coated with type I collagen was also examined using fluorescent immunostaining. Actin bundles were relatively thin and disorganized in endothelial cells seeded on SIS. Thick actin filaments were found at the periphery of the cells seeded on glass slides coated with type I collagen. This different organization could be due to the porosity, composition and hydrophilicity of the biomaterials used. During cell inflammation, monocytes were found adhering to ECs in the blood vessels causing ECs dysfunction. Activated leukocytes and macrophages release proinflammatory cytokines such as tumor necrosis factor-alpha (TNF-alpha) to enable ECs to increase their expression of adhesion molecules. In addition, TNF-alpha also causes ECs to produce vasodilators to increase blood flow to the affected area. Monocytes adhesion to endothelial cells was most likely the consequences of inflammation that might lead to the graft rejection. Human monocytes THP-1 cells were used to study the adhesion assay of monocytes to ECs seeded on SIS and glass slides coated with Type I collagen using fluorescent microscope. When activated with TNF-alpha, THP-1 cells showed more prominent adhesion to ECs cultured on glass slides coated with Type I collagen as compared to SIS. This result suggested that TNF-alpha induced higher number of endothelial cells-monocytes interaction on glass slides coated with Type I collagen compared to SIS.
Starting Page	290
Ending Page	295
File Size	744826
Page Count	6
File Format	PDF
ISBN	9789810579
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2006-12-11
Publisher Place	Singapore
Access Restriction	Subscribed
Rights Holder	NTU
Subject Keyword	Scanning electron microscopy Intestines Adhesives Shape Morphology Cells (biology) Glass Blood vessels Fluorescence In vitro
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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