NDLI: Analysis of promoter binding proteins of Ebp1 that is inhibitor protein of influenza virus RNA polymerase

Content Provider	IEEE Xplore Digital Library
Author	Mukai, M. Honda, A.
Copyright Year	2009
Description	Author affiliation: Hosei University (Mukai, M.; Honda, A.)
Abstract	Ebp1 is a member of the PA2G4 family protein and initially isolated as an ErB3 (an epidermal receptor tyrosine kinase) binding protein. Ebp1 inhibits cell growth and repress transcription of E2F-regulated cell cycle genes. Previously, we reported that Ebp1 protein interacted with RNA polymerase subunit PB1 of the influenza virus and disturbed in vitro RNA synthesis by influenza RNA polymerase. Recently we found that after influenza virus infection to the cell, Ebp1 expression is induced at 4 hours after viral infection. By using reverse genetics method, Ebp1 inhibits influenza virus replication. Ebp1 expression mechanism is very interesting because the expression of Ebp1 is earlier than that of virus proteins. In addition, in uninfection cell, Ebp1 is expressed from G1 to S phase in cell cycle-dependent manner. Therefore we study to identify the transcription factor of Ebp1 and understand the Ebp1 expression mechanism by influenza viral infection. Ebp1 promoter region was cloned into pTurboGFP. A pTurboGFP-Ebp1 promoter plasmid was mixed with nucleus extract form infection / uninfection cell. The binding proteins were eluted and analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). We identified several candidate proteins.
Starting Page	271
Ending Page	274
File Size	303616
Page Count	4
File Format	PDF
ISBN	9781424450947
DOI	10.1109/MHS.2009.5351934
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2009-11-09
Publisher Place	Japan
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Proteins Inhibitors Influenza RNA Polymers Epidermis Amino acids In vitro Genetics Mass spectroscopy
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Ebp1 expression is induced by influenza virus infection

The N-terminal region of influenza virus polymerase PB1 adjacent to the PA binding site is involved in replication but not transcription of the viral genome

Crystallization and X-ray diffraction analysis of the RNA primer/promoter-binding domain of influenza A virus RNA-dependent RNA polymerase PB2.

The Influenza Virus RNA-Polymerase and the Host RNA-Polymerase II: RPB4 Is Targeted by a PB2 Domain That Is Involved in Viral Transcription

Crystallization and X-ray diffraction analysis of the RNA primer/promoter-binding domain of influenza A virus RNA-dependent RNA polymerase PB2

Binding Cap Binding , and Virion RNA Promoter in Protein Stability , Endonuclease Activity , Virus RNA Polymerase Play a Critical Role Region of the PA Subunit of Influenza A Amino Acid Residues in the N-Terminal

A double-stranded RNA platform is required for the interaction between a host restriction factor and the NS1 protein of influenza A virus

Promoter analysis of influenza virus RNA polymerase.

Control of influenza virus growth by cellular proteins

Analysis of promoter binding proteins of Ebp1 that is inhibitor protein of influenza virus RNA polymerase

Similar Documents

Ebp1 expression is induced by influenza virus infection

The N-terminal region of influenza virus polymerase PB1 adjacent to the PA binding site is involved in replication but not transcription of the viral genome

Crystallization and X-ray diffraction analysis of the RNA primer/promoter-binding domain of influenza A virus RNA-dependent RNA polymerase PB2.

The Influenza Virus RNA-Polymerase and the Host RNA-Polymerase II: RPB4 Is Targeted by a PB2 Domain That Is Involved in Viral Transcription

Crystallization and X-ray diffraction analysis of the RNA primer/promoter-binding domain of influenza A virus RNA-dependent RNA polymerase PB2

Binding Cap Binding , and Virion RNA Promoter in Protein Stability , Endonuclease Activity , Virus RNA Polymerase Play a Critical Role Region of the PA Subunit of Influenza A Amino Acid Residues in the N-Terminal

A double-stranded RNA platform is required for the interaction between a host restriction factor and the NS1 protein of influenza A virus

Promoter analysis of influenza virus RNA polymerase.

Control of influenza virus growth by cellular proteins

Analysis of promoter binding proteins of Ebp1 that is inhibitor protein of influenza virus RNA polymerase