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Promoter analysis of influenza virus RNA polymerase.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Parvin, Jeffrey D. Palese, P. Honda, Ayae Ishihama, Akira Krystal, Mark |
| Copyright Year | 1989 |
| Abstract | Influenza virus polymerase, which was prepared depleted of viral RNA, was used to copy small RNA templates prepared from plasmid-encoded sequences. Template constructions containing only the 3' end of genomic RNA were shown to be efficiently copied, indicating that the promoter lay solely within the 15-nucleotide 3' terminus. Sequences not specific for the influenza virus termini were not copied, and, surprisingly, RNAs containing termini identical to those from plus-sense cRNA were copied at low levels. The specificity for recognition of the virus sense promoter was further defined by site-specific mutagenesis. It was also found that increased levels of viral protein were required in order to catalyze both the cap endonuclease-primed and primer-free RNA synthesis from these model templates, as well as from genomic-length RNAs. This finding indicates that the reconstituted system has catalytic properties very similar to those of native viral ribonucleoprotein complexes. |
| File Format | PDF HTM / HTML |
| DOI | 10.1128/jvi.63.12.5142-5152.1989 |
| PubMed reference number | 2585601 |
| Journal | Medline |
| Volume Number | 63 |
| Issue Number | 12 |
| Alternate Webpage(s) | http://jvi.asm.org/content/63/12/5142.full.pdf |
| Alternate Webpage(s) | https://doi.org/10.1128/jvi.63.12.5142-5152.1989 |
| Journal | Journal of virology |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
