NDLI: Crystallization and X-ray diffraction analysis of the RNA primer/promoter-binding domain of influenza A virus RNA-dependent RNA polymerase PB2.

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Crystallization and X-ray diffraction analysis of the RNA primer/promoter-binding domain of influenza A virus RNA-dependent RNA polymerase PB2.

Content Provider	Europe PMC
Author	Kuzuhara, Takashi Kise, Daisuke Yoshida, Hiroko Horita, Takahiro Murazaki, Yoshimi Utsunomiya, Hiroko Tsuge, Hideaki
Copyright Year	2009
Description	The C-terminal domain protein of the PB2 subunit of influenza A virus RNA-dependent RNA polymerase was expressed and crystallized and diffraction data were obtained from the crystals. The C-terminal domain protein (amino-acid residues 535–759) of the PB2 subunit of the RNA-dependent RNA polymerase from the highly pathogenic influenza A virus was expressed as a soluble protein in Escherichia coli and crystallized using sodium formate as a precipitant. Data sets were collected from crystals of native and selenomethionine-substituted protein on the KEK NW12 beamline at the Photon Factory and the crystals diffracted to a maximum resolution of 2.44 Å for the SeMet-derivative crystal. The native crystals were found to belong to space group P3221, with unit-cell parameters a = b = 52.5, c = 156.3 Å. The Matthews value (V M) was 2.7 Å3 Da−1, assuming the presence of one molecule in the asymmetric unit. The SeMet-derivative crystals were found to belong to the same space group, with unit-cell parameters a = b = 52.6, c = 156.4 Å. Attempts are being made to solve the structure by multi-wavelength anomalous dispersion phasing.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC2635877&blobtype=pdf
Volume Number	65
PubMed Central reference number	PMC2635877
Issue Number	Pt 2
Issue Number	pt 2
PubMed reference number	19194006
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications [Acta Crystallogr Sect F Struct Biol Cryst Commun]
e-ISSN	17443091
DOI	10.1107/s1744309108042942
Language	English
Publisher	International Union of Crystallography
Publisher Date	2009-01-31
Access Restriction	Open
Rights License	© International Union of Crystallography 2009
Subject Keyword	influenza A virus RNA-dependent RNA polymerase PB2
Content Type	Text
Resource Type	Article
Subject	Biochemistry Condensed Matter Physics Genetics Biophysics Structural Biology

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