NDLI: Position estimation of fluorescent probes in a confocal microscope

Content Provider	IEEE Xplore Digital Library
Author	Andersson, S.B.
Copyright Year	2007
Description	Author affiliation: Boston Univ., Boston (Andersson, S.B.)
Abstract	Measurements of fluorescence intensity from an isolated fluorescing probe in a confocal microscope can be viewed as range-only measurements of the position of that probe. We present an analytical algorithm for determining its position from a collection of measurements taken at different locations in the plane. This algorithm, fluoroBancroft, is inspired by the Bancroft algorithm in GPS. We investigate the performance of the algorithm through simulation and compare it to the standard technique of fitting the data to a Gaussian profile. Our results indicate that the fluoroBancroft algorithm is more accurate than the fitting procedure when only a few measurements are used. Moreover the technique is typically two orders of magnitude faster in terms of computation time. These preliminary results indicate the fluoroBancroft algorithm holds promise for use in a real-time closed-loop controller to track the motion of single fluorescent probes.
Starting Page	4950
Ending Page	4955
File Size	1264352
Page Count	6
File Format	PDF
ISBN	9781424414970
ISSN	01912216
DOI	10.1109/CDC.2007.4434907
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2007-12-12
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fluorescence Probes Position measurement Global Positioning System Algorithm design and analysis Particle tracking Ultrafast optics Optical microscopy USA Councils Computational modeling
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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