NDLI: Real-time detection of CTL function reveals distinct patterns of caspase activation mediated by Fas versus granzyme B.

Please wait, while we are loading the content...

Year: 2017 Volume: 198

Year: 2016 Volume: 197

Year: 2016 Volume: 196

Year: 2015 Volume: 195

Year: 2015 Volume: 194

Year: 2014 Volume: 193

Year: 2014 Volume: 193 Issue: 12

Year: 2014 Volume: 193 Issue: 11

Year: 2014 Volume: 193 Issue: 10

Year: 2014 Volume: 193 Issue: 9

Year: 2014 Volume: 193 Issue: 8

Year: 2014 Volume: 193 Issue: 7

Year: 2014 Volume: 193 Issue: 6

Year: 2014 Volume: 193 Issue: 5

Year: 2014 Volume: 193 Issue: 4

Year: 2014 Volume: 193 Issue: 3

Year: 2014 Volume: 193 Issue: 2

Themis2 is not required for B cell development, activation, and antibody responses.

Early lymphoid responses and germinal center formation correlate with lower viral load set points and better prognosis of simian immunodeficiency virus infection.

The peritrophic matrix mediates differential infection outcomes in the tsetse fly gut following challenge with commensal, pathogenic, and parasitic microbes.

Targeted delivery of -galactosylceramide to CD8 + dendritic cells optimizes type I NKT cell-based antitumor responses.

Immune memory-boosting dose of rapamycin impairs macrophage vesicle acidification and curtails glycolysis in effector CD8 cells, impairing defense against acute infections.

Cytokine-regulated neutrophil recruitment is required for brain but not spinal cord inflammation during experimental autoimmune encephalomyelitis.

Reactive oxygen species- and DNA damage response-dependent NK cell activating ligand upregulation occurs at transcriptional levels and requires the transcriptional factor E2F1.

Dual function of CD70 in viral infection: modulator of early cytokine responses and activator of adaptive responses.

IL-17 promotes murine lupus.

Id1 expression promotes T regulatory cell differentiation by facilitating TCR costimulation.

Elevated levels of mast cells are involved in pruritus associated with polycythemia vera in JAK2V617F transgenic mice.

Cellular stress amplifies TLR3/4-induced CXCL1/2 gene transcription in mononuclear phagocytes via RIPK1.

Acute and chronic B cell depletion disrupts CD4+ and CD8+ T cell homeostasis and expansion during acute viral infection in mice.

Site-specific chemokine expression regulates central nervous system inflammation and determines clinical phenotype in autoimmune encephalomyelitis.

Ficolin-2 inhibits hepatitis C virus infection, whereas apolipoprotein E3 mediates viral immune escape.

CD40 ligand preferentially modulates immune response and enhances protection against influenza virus.

Real-time detection of CTL function reveals distinct patterns of caspase activation mediated by Fas versus granzyme B.

B cells regulate CD4+ T cell responses to papain following B cell receptor-independent papain uptake.

Adventitial fibroblasts induce a distinct proinflammatory/profibrotic macrophage phenotype in pulmonary hypertension.

Investigation of soluble and transmembrane CTLA-4 isoforms in serum and microvesicles.

Innate PLZF+CD4+ ß T cells develop and expand in the absence of Itk.

High proportion of CD5+ B cells in infants predicts development of allergic disease.

Macrophage migration inhibitory factor promotes clearance of pneumococcal colonization.

Rituximab efficiently depletes increased CD20-expressing T cells in multiple sclerosis patients.

ERAP1-ERAP2 dimerization increases peptide-trimming efficiency.

Targeting of TLRs inhibits CD4+ regulatory T cell function and activates lymphocytes in human peripheral blood mononuclear cells.

A balance between BCR and inhibitory receptor signaling controls plasma cell differentiation by maintaining optimal Ets1 levels

Opposing roles of prostaglandin D2 receptors in ulcerative colitis.

The Wnt/ß-catenin pathway attenuates experimental allergic airway disease.

DOK3 is required for IFN-ß production by enabling TRAF3/TBK1 complex formation and IRF3 activation.

Apurinic/apyrimidinic endonuclease 2 regulates the expansion of germinal centers by protecting against activation-induced cytidine deaminase-independent DNA damage in B cells.

Human NKG2E is expressed and forms an intracytoplasmic complex with CD94 and DAP12.

Adrenocortical scavenger receptor class B type I deficiency exacerbates endotoxic shock and precipitates sepsis-induced mortality in mice.

Tryptophan catabolism restricts IFN-Î³-expressing neutrophils and Clostridium difficile immunopathology.

PILR negatively regulates mouse inflammatory arthritis.

Molecular determinants of agonist and antagonist signaling through the IL-36 receptor.

Human NK cells licensed by killer Ig receptor genes have an altered cytokine program that modifies CD4+ T cell function.

Autotaxin produced by stromal cells promotes LFA-1-independent and Rho-dependent interstitial T cell motility in the lymph node paracortex.

Inhibition of PI3KÎ´ reduces kidney infiltration by macrophages and ameliorates systemic lupus in the mouse.

gp49B-mediated negative regulation of antibody production by memory and marginal zone B cells.

Autologous tumor vaccine modified with recombinant new castle disease virus expressing IL-7 promotes antitumor immune response.

Development of innate CD4+ and CD8+ T cells in Itk-deficient mice is regulated by distinct pathways.

Humanized mice as a model for aberrant responses in human T cell immunotherapy.

TCR affinity and tolerance mechanisms converge to shape T cell diabetogenic potential.

Regulatory T cells restrain CD4+ T cells from causing unregulated immune activation and hypersensitivity to lipopolysaccharide challenge.

GM-CSF-licensed CD11b+ lung dendritic cells orchestrate Th2 immunity to Blomia tropicalis.

Human Th2 but not Th9 cells release IL-31 in a STAT6/NF-κB-dependent way.

Metabolic engineering of Salmonella vaccine bacteria to boost human VÎ³2VÎ´2 T cell immunity.

GM-CSF but not IL-17 is critical for the development of severe interstitial lung disease in SKG mice.

Comment on 'Tim-3 directly enhances CD8 T cell responses to acute Listeria monocytogenes infection'.

Influenza-specific antibody-dependent cellular cytotoxicity: toward a universal influenza vaccine.

Year: 2014 Volume: 193 Issue: 1

Year: 2014 Volume: 192

Year: 2013 Volume: 191

Year: 2013 Volume: 190

Year: 2012 Volume: 189

Year: 2012 Volume: 188

Year: 2011 Volume: 187

Year: 2011 Volume: 186

Year: 2010 Volume: 185

Year: 2010 Volume: 184

Year: 2009 Volume: 183

Year: 2009 Volume: 182

Year: 2008 Volume: 181

Real-time detection of CTL function reveals distinct patterns of caspase activation mediated by Fas versus granzyme B.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Jordan, Michael B. Tabata, Yasuhiro Figueira, Sarah K. Butler, Braeden L. Filipovich, Alexandra Li, Jinzhu Binkowski, Brock F. Risma, Kimberly A. Vrazo, Alexandra C. A.
Description	Author Affiliation: Li J ( Division of Allergy/Immunology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229); Figueira SK ( Division of Allergy/Immunology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229); Vrazo AC ( Division of Allergy/Immunology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229); Binkowski BF ( Promega Corp., Madison, WI 53704); Butler BL ( Promega Corp., Madison, WI 53704); Tabata Y ( Division of Bone Marrow Transplantation and Immune Deficiency, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229); Filipovich A ( Division of Bone Marrow Transplantation and Immune Deficiency, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229); Jordan MB ( Division of Bone Marrow Transplantation and Immune Deficiency, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229); Risma KA ( Division of Allergy/Immunology, Cincinnati Children's Hospital Medical Center, Cincinnati, OH 45229)
Abstract	Activation of caspase-mediated apoptosis is reported to be a hallmark of both granzyme B- and Fas-mediated pathways of killing by CTLs; however, the kinetics of caspase activation remain undefined owing to an inability to monitor target cell-specific apoptosis in real time. We have overcome this limitation by developing a novel biosensor assay that detects continuous, protease-specific activity in target cells. Biosensors were engineered from a circularly permuted luciferase, linked internally by either caspase 3/7 or granzyme B/caspase 8 cleavage sites, thus allowing activation upon proteolytic cleavage by the respective proteases. Coincubation of murine CTLs with target cells expressing either type of biosensor led to a robust luminescent signal within minutes of cell contact. The signal was modulated by the strength of TCR signaling, the ratio of CTL/target cells, and the type of biosensor used. Additionally, the luciferase signal at 30 min correlated with target cell death, as measured by a (51)Cr-release assay. The rate of caspase 3/7 biosensor activation was unexpectedly rapid following granzyme B- compared with Fas-mediated signal induction in murine CTLs; the latter appeared gradually after a 90-min delay in perforin- or granzyme B-deficient CTLs. Remarkably, the Fas-dependent, caspase 3/7 biosensor signal induced by perforin-deficient human CTLs was also detectable after a 90-min delay when measured by redirected killing. Thus, we have used a novel, real-time assay to demonstrate the distinct pattern of caspase activation induced by granzyme B versus Fas in human and murine CTLs.
ISSN	00221767
e-ISSN	15506606
DOI	10.4049/jimmunol.1301668
Journal	The Journal of Immunology
Issue Number	2
Volume Number	193
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2014-07-15
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Antigens, Cd95 Immunology Caspases Granzymes T-lymphocytes, Cytotoxic Animals Metabolism Apoptosis Binding Sites Genetics Caspase 3 Caspase 7 Cell Line, Tumor Cytotoxicity Tests, Immunologic Cytotoxicity, Immunologic Enzyme Activation Luciferases Luminescent Measurements Mice Mice, Inbred C57bl Mice, Knockout Mice, Transgenic Perforin Signal Transduction Time Factors Research Support, N.i.h., Extramural Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in