NDLI: Autologous tumor vaccine modified with recombinant new castle disease virus expressing IL-7 promotes antitumor immune response.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Mei, Yu Sun, Qing Liu, Xiufan Wu, Yan Guo, Linghua Liu, Haiyan Zhao, Lixiang Yu, Xiao Hu, Bo
Description	Author Affiliation: Zhao L ( Laboratory of Cellular and Molecular Tumor Immunology, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China); Mei Y ( Laboratory of Cellular and Molecular Tumor Immunology, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China); Sun Q ( Animal Infectious Disease Laboratory, School of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China); Guo L ( Laboratory of Cellular and Molecular Tumor Immunology, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China); Wu Y ( Laboratory of Cellular and Molecular Tumor Immunology, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China); Yu X ( Laboratory of Cellular and Molecular Tumor Immunology, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China); Hu B ( Laboratory of Cellular and Molecular Tumor Immunology, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China); Liu X ( Animal Infectious Disease Laboratory, School of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China); Liu H ( Laboratory of Cellular and Molecular Tumor Immunology, Institutes of Biology and Medical Sciences, Soochow University, Suzhou 215123, China)
Abstract	Autologous tumor vaccine modified with nonlytic Newcastle disease virus (ATV-NDV) is a promising vaccine for cancer immunotherapy. IL-7 plays a critical role in lymphocyte development and homeostasis. To improve the efficacy of ATV-NDV, we inserted the murine IL-7 gene into the genome of nonlytic NDV strain LX using reverse genetic system. The insertion of the IL-7 gene neither affected the main features of NDV replication nor its tumor selectivity. The gene product was biologically active and stable. Then we tested the antitumor effects of the autologous tumor vaccine modified with LX/(IL-7) in the murine tumor models. We showed that tumor cells modified with LX/IL-7 induced a strong antitumor activity both in prophylaxis and therapeutic models. The IFN-Î³ production and the cytotoxicity of tumor-specific CD8(+) T cells were significantly enhanced after immunization with tumor cells modified with LX/(IL-7) in both models. Although the tumor-infiltrating CD4(+) T cells and CD8(+) T cells were both increased and their IFN-Î³ productions also were upregulated, the antitumor activity of the tumor vaccine modified with LX/(IL-7) was dependent on CD8(+) T cells. Our results demonstrated that the autologous tumor vaccine modified with NDV strain LX/(IL-7) could promote the antitumor immune responses mediated by CD8(+) T cells and significantly improve the efficacy of the ATV-NDV.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	2
Volume Number	193
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2014-07-15
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Antigens, Neoplasm Immunology Cancer Vaccines Interleukin-7 Neoplasms, Experimental Newcastle Disease Virus Animals Genetics Cd4-positive T-lymphocytes Metabolism Cd8-positive T-lymphocytes Cell Line, Tumor Cytotoxicity, Immunologic Dna, Recombinant Flow Cytometry Immunotherapy Interferon-gamma Mice Mice, Inbred C57bl Pathology Therapy Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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