NDLI: Single-cell perforin and granzyme expression reveals the anatomical localization of effector CD8+ T cells in influenza virus-infected mice.

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Single-cell perforin and granzyme expression reveals the anatomical localization of effector CD8+ T cells in influenza virus-infected mice.

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Single-cell perforin and granzyme expression reveals the anatomical localization of effector CD8+ T cells in influenza virus-infected mice.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Costelloe, Elaine O. Fitzpatrick, David R. Brown, Lorena E. Schumacher, Ton N. M. Johnson, Barbara J. Haanen, John B. A. G. Kelso, Anne
Description	Author Affiliation: Johnson BJ ( Cooperative Research Centre for Vaccine Technology and Queensland Institute of Medical Research, Post Office Royal Brisbane Hospital, Queensland 4029, Australia.);
Abstract	Influenza virus infection activates cytolytic T lymphocytes (CTL) that contribute to viral clearance by releasing perforin and granzymes from cytoplasmic granules. Virus-specific, perforin-dependent CD8(+) CTL were detected in freshly isolated cells from the mouse lung parenchyma but not from the mediastinal lymph nodes (MLN), where they are primed, or from the spleen during primary influenza virus infection. To determine whether this difference was due to the low frequency or incomplete maturation of effector CTL in MLN, we measured expression of perforin, granzymes A, B, and C, and IFN-gamma mRNAs in CD8(+) populations and single cells immediately after isolation from virus-infected mice. Quantitative PCR revealed significant expression of perforin, granzyme A, granzyme B, and IFN-gamma in activated CD8(+) cells from MLN, spleen, and lung parenchyma. Granzyme C expression was not detected. Individual activated or nucleoprotein peptide/class I tetramer-binding CD8(+) cells from the three tissues expressed diverse combinations of perforin, granzyme, and IFN-gamma mRNAs. Although cells from lung expressed granzymes A and B at higher frequency, each of the tissues contained cells that coexpressed perforin with granzymes A and/or B. The main difference between MLN and lung was the elevated frequency of activated CD8(+) T cells in the lung, rather than their perforin/granzyme expression profile. The data suggest that some CTL mature into perforin/granzyme-expressing effector cells in MLN but reach detectable frequencies only when they accumulate in the infected lung.
ISSN	00278424
e-ISSN	10916490
Journal	Proceedings of the National Academy of Sciences of the United States of America
Issue Number	5
Volume Number	100
Language	English
Publisher	National Academy of Sciences
Publisher Date	2003-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	CD8-Positive T-Lymphocytes Metabolism Membrane Glycoproteins Biosynthesis Orthomyxoviridae Serine Endopeptidases Animals Antigens, CD44 Antigens, CD8 Immunology Cells, Cultured Chromium Radioisotopes Comet Assay Granzymes Interferon-gamma Lung Lymph Nodes Mice Mice, Inbred BALB C Mice, Inbred C57BL Perforin Phenotype Pore Forming Cytotoxic Proteins RNA, Messenger Reverse Transcriptase Polymerase Chain Reaction Spleen Cytology T-Lymphocytes T-Lymphocytes, Cytotoxic Research Support, Non-U.S. Gov't Multidisciplinary
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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