NDLI: Two-photon imaging of the cornea visualized in the living mouse using vital dyes.

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Two-photon imaging of the cornea visualized in the living mouse using vital dyes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhang, Junjie Zhang, Hongmin Liu, Susu Li, Zhijie Sun, Shengtao Xie, Yanting He, Siyu Li, Xiaohua Wang, Liya Deng, Xianming
Description	Author Affiliation: Zhang H ( Henan Eye Institute, Henan Eye Hospital, Department of Ophthalmology, Henan Provincial People's Hospital and People's Hospital of Zhengzhou University, Zhengzhou, People's Republic of China.)
Abstract	PURPOSE: To acquire morphological and component information on the overall cornea in the living C57BL/6 mouse using fluorescent viability dyes and two-photon (2PH) laser microscopy. METHODS: Corneas were scanned using a 2PH laser scanning fluorescence microscope after staining with plasma membrane stain and Hoechst 33342. Representative 2PH images of corneal cells were analyzed and restructured using Imaris software. RESULTS: With the plasma membrane- and cell-permeant nuclear counter live cell fluorescent probe, the morphology of corneal cells and the construction of cornea were observed clearly. The general in vivo morphology of the cornea clearly showed three different cellular layers, two interfaces, and the nerve fibers. Our study detailed all of the corneal cells with clear cell nuclei and cell boundaries at the sections parallel to the surface of the cornea. Moreover, cellular stereoscopic images, the relationships of the neighbor cells, and the interfaces of different layers were also displayed distinctly with three-dimensional construction of the 2PH imaging. Our research showed for the first time that there are three or four layers of epithelial cells and seven or eight layers of keratocytes in mice. CONCLUSIONS: Our study clearly showed the anterior limiting lamina and Descemet's membrane in the mouse cornea and showed for the first time that there are three or four layers of cells in the epithelium and seven or eight layers of keratocytes in the stroma of mice. These results are necessary primarily to contribute important insights into the anatomy and pathology of the cornea in mice.
ISSN	01460404
e-ISSN	15525783
Journal	Investigative Opthalmology & Visual Science
Issue Number	10
Volume Number	54
Language	English
Publisher	Association for Research in Vision and Ophthalmology
Publisher Date	2013-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Cornea Cytology Fluorescent Dyes Photons Animals Cell Count Imaging, Three-dimensional Mice Mice, Inbred C57bl Microscopy, Confocal Nerve Fibers Comparative Study Research Support, Non-u.s. Gov't Discipline Ophthalmology
Content Type	Text
Resource Type	Article
Subject	Ophthalmology Sensory Systems Cellular and Molecular Neuroscience

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