NDLI: Tear cytokine profile as a noninvasive biomarker of inflammation for ocular surface diseases: standard operating procedures.

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Tear cytokine profile as a noninvasive biomarker of inflammation for ocular surface diseases: standard operating procedures.

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Tear cytokine profile as a noninvasive biomarker of inflammation for ocular surface diseases: standard operating procedures.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Asbell, Penny Wei, Yi Epstein, Seth Gadaria-Rathod, Neha
Description	Author Affiliation: Wei Y ( Department of Ophthalmology, Icahn School of Medicine at Mount Sinai, New York, New York.)
Abstract	PURPOSE: To provide standard operating procedures (SOPs) for measuring tear inflammatory cytokine concentrations and to validate the resulting profile as a minimally invasive objective metric and biomarker of ocular surface inflammation for use in multicenter clinical trials on dry eye disease (DED). METHODS: Standard operating procedures were established and then validated with cytokine standards, quality controls, and masked tear samples collected from local and distant clinical sites. The concentrations of the inflammatory cytokines in tears were quantified using a high-sensitivity human cytokine multiplex kit. RESULTS: A panel of inflammatory cytokines was initially investigated, from which four key inflammatory cytokines (IL-1ß, IL-6, INF-Î³, and TNF- ) were chosen. Results with cytokine standards statistically satisfied the manufacturer's quality control criteria. Results with pooled tear samples were highly reproducible and reliable with tear volumes ranging from 4 to 10 µL. Incorporation of the SOPs into clinical trials was subsequently validated. Tear samples were collected at a distant clinical site, stored, and shipped to our Biomarker Laboratory, where a masked analysis of the four tear cytokines was successfully performed. Tear samples were also collected from a feasibility study on DED. Inflammatory cytokine concentrations were decreased in tears of subjects who received anti-inflammatory treatment. CONCLUSIONS: Standard operating procedures for human tear cytokine assessment suitable for multicenter clinical trials were established. Tear cytokine profiling using these SOPs may provide objective metrics useful for diagnosing, classifying, and analyzing treatment efficacy in inflammatory conditions of the ocular surface, which may further elucidate the mechanisms involved in the pathogenesis of ocular surface disease.
ISSN	01460404
e-ISSN	15525783
DOI	10.1167/iovs.13-12132
Journal	Investigative Opthalmology & Visual Science
Issue Number	13
Volume Number	54
Language	English
Publisher	Association for Research in Vision and Ophthalmology
Publisher Date	2013-12-23
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Biological Markers Metabolism Cytokines Dry Eye Syndromes Inflammation Double-blind Method Interferon-gamma Interleukin-1beta Interleukin-6 Reproducibility Of Results Sensitivity And Specificity Questionnaires Tumor Necrosis Factor-alpha Multicenter Study Randomized Controlled Trial Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Discipline Ophthalmology
Content Type	Text
Resource Type	Article
Subject	Ophthalmology Sensory Systems Cellular and Molecular Neuroscience

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