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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Pereira, R. W. Nogueira, L. R. Gentil, P. Oliveira, R. J. Lins, T. C. Lima, R. M. |
| Spatial Coverage | Brazil |
| Description | Country affiliation: Brazil Responsible library: BR1.1 Author Affiliation: Lins, T. C ( Universidade Católica de Brasília. Brasília. BR); Pereira, R. W ( Universidade Católica de Brasília. Brasília. BR); Oliveira, R. J ( Universidade Católica de Brasília. Brasília. BR); Gentil, P ( Universidade Católica de Brasília. Brasília. BR); Lima, R. M ( Universidade Católica de Brasília. Brasília. BR); Nogueira, L. R ( Universidade Católica de Brasília. Brasília. BR) |
| Abstract | The well-described role of the vitamin D endocrine system in bone metabolism makes its receptor a widely investigated candidate gene in association studies looking for the genetic basis of complex bone-related phenotypes. Most association studies genotype five polymorphic sites along the gene using PCR-RFLP and allele-specific amplification methods, which may not be the better choice in large case/control or cross-sectional studies. In this case, genotyping SNPs in parallel and using automated allele-calling methods are important to decrease genotyping errors due to manual data handling and save sample in cases where the amount of DNA is limited. The aim of this study was to present a straightforward method based on multiplex PCR amplification followed by multiplex single-base extension as a simple way to genotype five vitamin D receptor gene polymorphisms in parallel, which may be implemented in medium- to large-scale case/control or cross-sectional studies. The results regarding method feasibility and optimization are presented by genotyping eight paternity trios and seven samples of Brazilian postmenopausal women who took part in an ongoing association study carried out by members of our group. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 2 |
| Volume Number | 6 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Genotype Polymerase Chain Reaction Polymorphism, Single Nucleotide Receptors, Calcitriol Genetics Electrophoresis, Capillary Postmenopause Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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