NDLI: Description of electrophoretic and chromatographic hemoglobin profile of Rhinoclemmys punctularia

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Description of electrophoretic and chromatographic hemoglobin profile of Rhinoclemmys punctularia

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Description of electrophoretic and chromatographic hemoglobin profile of Rhinoclemmys punctularia

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Moreira, S. B. Zago, C. E. Zamaro, P. J. Romero, R. M. Bonini-Domingos, C. R. Silva, M. B. Salgado, C. G. Ondei, L. S.
Description	Country affiliation: Brazil Responsible library: BR1.1 Author Affiliation: Bonini-Domingos, C. R ( UNESP. IBILCE. Departamento de Biologia. Laboratório de Hemoglobinas e Genética das Doenças Hematológicas. São José do Rio Preto. BR); Salgado, C. G ( UEPA. Laboratório de Dermatoimunologia. Belém. BR); Moreira, S. B ( Museu Paraense Emilio Goeldi. Belém. BR); Zago, C. E ( UNESP. IBILCE. Departamento de Biologia. Laboratório de Hemoglobinas e Genética das Doenças Hematológicas. São José do Rio Preto. BR); Ondei, L. S ( UNESP. IBILCE. Departamento de Biologia. Laboratório de Hemoglobinas e Genética das Doenças Hematológicas. São José do Rio Preto. BR); Zamaro, P. J ( UNESP. IBILCE. Departamento de Biologia. Laboratório de Hemoglobinas e Genética das Doenças Hematológicas. São José do Rio Preto. BR); Romero, R. M ( UNESP. IBILCE. Departamento de Biologia. Laboratório de Hemoglobinas e Genética das Doenças Hematológicas. São José do Rio Preto. BR); Silva, M. B ( UNESP. IBILCE. Departamento de Biologia. Laboratório de Hemoglobinas e Genética das Doenças Hematológicas. São José do Rio Preto. BR)
Abstract	Studies of the hemoglobin pattern in Brazilian reptiles are important for determining ecological and phylogenetic relationships, but they are scarce. Peripheral blood samples were obtained from 7 males and 18 females of Rhinoclemmys punctularia. The hematological profile was based on the total hemoglobin and hematocrit values. The hemoglobin profile was obtained using electrophoretic procedures at different pH, isoelectric focusing, globin chain electrophoresis, and HPLC. The hematocrit (31 +/- 2%) and total hemoglobin (7.5 +/- 0.2 g/dL) values did not indicate gender variations. Alkaline pH electrophoresis of the total blood samples treated with 1% saponin demonstrated the presence of four well-defined hemoglobin fractions, one major component (fraction I), showing cathodic migration and three others faster than fraction I with anodic migration. When the samples were precipitated with chloroform, only two hemoglobin fractions were observed, similar to fractions I and III from the first procedure. Isoelectric focusing and HPLC showed the same pattern. With acid and neutral pH electrophoresis, two fractions with anodic migration were observed. The globin chain identification at alkaline pH showed two fractions, but four fractions were observed at acidic pH, suggesting that different polypeptide chains are involved in the hemoglobin molecule. The chromatographic separation of the total blood sample demonstrated that the major fraction comprised 81.9% and the minor 18.1%. The results obtained demonstrated a similarity between these hemoglobin components and those of some Chelidae reported in the literature for both land and aquatic animals, reflecting the adaptation to environmental conditions.
e-ISSN	16765680
Journal	Genetics and Molecular Research
Issue Number	2
Volume Number	6
Language	English
Publisher	Fundação de Pesquisas Científicas de Ribeirão Preto
Publisher Date	2007-01-01
Publisher Place	Brazil
Access Restriction	Open
Subject Keyword	Animals Chromatography Electrophoresis Hemoglobins Genetics Metabolism Polymorphism, Genetic Turtles Ecology Hematocrit Models, Genetic Phylogeny Reptiles Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics
Content Type	Text
Resource Type	Article

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