NDLI: Genetic variability in maned wolf based on heterologous short-tandem repeat markers from domestic dog

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Genetic variability in maned wolf based on heterologous short-tandem repeat markers from domestic dog

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Genetic variability in maned wolf based on heterologous short-tandem repeat markers from domestic dog

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Akimoto, A. A. Salim, D. C. Moreira, J. R. Klautau-Guimarães, M. N. Oliveira, S. F. Grisolia, C. K. Carvalho, C. B.
Spatial Coverage	Brazil
Description	Country affiliation: Brazil Responsible library: BR1.1 Author Affiliation: Salim, D. C ( Universidade de Brasília. Instituto de Biologia. Departamento de Genética e Morfologia. Laboratório de Genética. Brasília. BR); Grisolia, C. K ( Universidade de Brasília. Instituto de Biologia. Departamento de Genética e Morfologia. Laboratório de Genética. Brasília. BR); Klautau-Guimarães, M. N ( Universidade de Brasília. Instituto de Biologia. Departamento de Genética e Morfologia. Laboratório de Genética. Brasília. BR); Moreira, J. R ( Embrapa Recursos Genéticos e Biotecnologia. Brasília. BR); Oliveira, S. F ( Universidade de Brasília. Instituto de Biologia. Departamento de Genética e Morfologia. Laboratório de Genética. Brasília. BR); Carvalho, C. B ( Universidade de Brasília. Instituto de Biologia. Departamento de Genética e Morfologia. Laboratório de Genética. Brasília. BR); Akimoto, A. A ( Universidade de Brasília. Instituto de Biologia. Departamento de Genética e Morfologia. Laboratório de Genética. Brasília. BR)
Abstract	The maned wolf (Chrysocyon brachyurus) is the largest South American canid. Habitat loss and fragmentation, due to agricultural expansion and predatory hunting, are the main threats to this species. It is included in the official list of threatened wildlife species in Brazil, and is also protected by IUCN and CITES. Highly variable genetic markers such as microsatellites have the potential to resolve genetic relationships at all levels of the population structure (among individuals, demes or metapopulations) and also to identify the evolutionary unit for strategies for the conservation of the species. Tests were carried out to verify whether a class of highly polymorphic tetranucleotide repeats described for the domestic dog effectively amplifies DNA in the maned wolf. All five loci studied were amplified; however, one of these, was shown to be monomorphic in 69 maned wolf samples. The average allele number and estimated heterozygosity per polymorphic locus were 4.3 and 67%, respectively. The genetic variability found for this species, which is considered threatened with extinction, showed similar results when compared to studies of other canids.
e-ISSN	16765680
Journal	Genetics and Molecular Research
Issue Number	2
Volume Number	6
Language	English
Publisher	Fundação de Pesquisas Científicas de Ribeirão Preto
Publisher Place	Brazil
Access Restriction	Open
Subject Keyword	Animals Genetic Markers Microsatellite Repeats Genetic Variation Alleles Genetics, Population Geography Polymorphism, Genetic Species Specificity Wolves Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics
Content Type	Text
Resource Type	Article

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